Miniature mass spectrometer-based point-of-care assay for measuring phosphatidylethanol in blood.

Miniature mass spectrometer-based point-of-care assay for measuring phosphatidylethanol in blood.
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DOI:
10.1039/d3an00098b
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发表时间:
2023-03-27
期刊:
The Analyst
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我们通过使用纳米电喷雾电离测量血液/干血斑中的磷脂酰乙醇,并使用微型质谱仪测量MS/MS,为长期酒精消费的护理点评估提供了概念验证。对于两种样本类型,“禁欲”、“适度”和“慢性”消费都可以迅速区分,并通过血液(LOQ-100ngmL−1)获得定量表现。
We demonstrate proof-of-concept for point-of-care assessment of long-term alcohol consumption by measuring phosphatidylethanol in blood/dried blood spots with nano-electrospray ionization and MS/MS using a miniature mass spectrometer. ‘Abstinence’, ‘moderate’, and ‘chronic’ consumption could be distinguished rapidly for both sample types, and quantitative performance was obtained with blood (LoQ-100 ng mL−1).
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