Ozone induces flavonol production of Ginkgo biloba cells dependently on nitrate reductase-mediated nitric oxide signaling
Ozone induces flavonol production of Ginkgo biloba cells dependently on nitrate reductase-mediated nitric oxide signaling
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臭氧诱导银杏叶细胞产生黄酮醇，依赖于硝酸还原酶介导的一氧化氮信号传导
DOI：
10.1016/j.envexpbot.2011.09.005
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发表时间：
2012
影响因子：
5.7
通讯作者：
Lu Dan
中科院分区：
文献类型：
--
作者：
Xu Maojun;Zhu Yun;Dong Jufang;Jin Haihong;Sun Lina;Wang Zhian;Lu Zhonghua;Lu Dan
次生代谢产物的积累和一氧化氮（NO）的产生是植物对臭氧暴露的两种早期反应。然而，在臭氧诱导的次生代谢产物积累和臭氧触发NO生成的来源NO的作用在很大程度上是未知的。在这里，我们报告，臭氧暴露诱导银杏细胞的黄酮醇积累和NO的产生。用NO特异性清除剂2-（4-羧基苯基）-4，4，5，5-四甲基咪唑啉-1-氧基-3-氧化物钾（cPTIO）预处理细胞，可抑制臭氧诱导的细胞黄酮醇的产生，表明NO是臭氧诱导的黄酮醇积累所必需的。此外，臭氧暴露显着增强硝酸还原酶（NR）的细胞活性。NR抑制剂的应用不仅抑制了臭氧引发的NR活性，而且抑制了臭氧诱导的NO生成，表明臭氧可能依赖于NR活性诱导NO生成。此外，NR抑制剂处理的细胞抑制臭氧诱导的黄酮醇的生产和NR抑制剂对臭氧诱导的黄酮醇的生产的抑制可以逆转通过其供体硝普钠（SNP）的外源性应用NO。总之，我们的研究结果表明NR介导的NO信号参与了臭氧诱导的G.二叶细胞
Accumulation of secondary metabolites and nitric oxide (NO) generation are two early responses of plants to ozone exposure. However, the role of NO in ozone-induced secondary metabolite accumulation and the source of ozone-triggered NO generation are largely unknown. Here, we report that ozone exposure induces flavonol accumulation and NO generation of Ginkgo biloba cells. Pretreatment of the cells with NO specific scavenger 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide potassium (cPTIO) inhibits the ozone-induced flavonol production of the cells, showing that NO is essential for ozone-induced flavonol accumulation. Moreover, ozone exposure significantly enhances nitrate reductase (NR) activity of the cells. Application of NR inhibitors not only suppresses ozone-triggered NR activity but also inhibits ozone-induced NO generation, showing that ozone may induce NO generation dependently on NR activity. Furthermore, treatment of the cells with NR inhibitors suppresses the ozone-induced flavonol production and the suppression of NR inhibitors on ozone-induced flavonol production can be reversed by exogenous application of NO via its donor sodium nitroprusside (SNP). Together, our results suggest that the NR-mediated NO signaling is involved in ozone-induced flavonol production of G. biloba cells.
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影响因子：
64.8
作者：
Delledonne, M;Xia, YJ;Lamb, C
通讯作者：
Lamb, C
影响因子：
--
作者：
M. Murphy;E. Noack
通讯作者：
M. Murphy;E. Noack
影响因子：
7.4
作者：
DEAN, JV;HARPER, JE
通讯作者：
HARPER, JE
DOI：
10.1111/j.1365-3040.2009.01976.x
发表时间：
2009-08
期刊：
Plant, cell & environment
影响因子：
--
作者：
Maojun Xu;Jufang Dong;Huizhong Wang;Luqi Huang
通讯作者：
Maojun Xu;Jufang Dong;Huizhong Wang;Luqi Huang
影响因子：
1.2
作者：
A. Yamamoto;S. Katou;H. Yoshioka;N. Doke;K. Kawakita
通讯作者：
A. Yamamoto;S. Katou;H. Yoshioka;N. Doke;K. Kawakita