Ancient association between cation leak channels and Mid1 proteins is conserved in fungi and animals.
Ancient association between cation leak channels and Mid1 proteins is conserved in fungi and animals.
复制标题
阳离子泄漏通道与MID1蛋白之间的古老关联在真菌和动物中保存。
DOI：
10.3389/fnmol.2014.00015
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发表时间：
2014
影响因子：
4.8
通讯作者：
Zakon HH
中科院分区：
文献类型：
--
作者：
Ghezzi A;Liebeskind BJ;Thompson A;Atkinson NS;Zakon HH
神经元静息电位可以调节神经网络的兴奋性，影响下游行为。钠漏通道（NALCN）通过帮助设置或微妙地改变神经元静息电位在节律行为中起关键作用。然而，这些新描述的通道的全部复杂性才刚刚开始被理解。NALCN通道可以与不同组织中的许多亚基缔合，并且可以被几种不同的肽和第二信使激活。我们最近发现NALCN通道与真菌钙通道密切相关，它们在功能上相似。在这里，我们使用这种关系来预测一个家庭的NALCN相关蛋白在动物的基础上同源性与酵母蛋白Mid1，酵母钙通道的亚基。这些蛋白质都有一个富含半胱氨酸的区域，这是酵母中Mid1功能所必需的。我们验证了这一预测的关联表明，在果蝇中的Mid1同源物，由CG33988基因编码，与NALCN协调表达，敲除任何一种蛋白质在与NALCN功能相关的几个行为中产生相同的表型。因此，Mid1和泄漏通道之间的关系持续了10亿年的进化，尽管蛋白质和它们存在的生物体发生了巨大的变化。
Neuronal resting potential can tune the excitability of neural networks, affecting downstream behavior. Sodium leak channels (NALCN) play a key role in rhythmic behaviors by helping set, or subtly changing neuronal resting potential. The full complexity of these newly described channels is just beginning to be appreciated, however. NALCN channels can associate with numerous subunits in different tissues and can be activated by several different peptides and second messengers. We recently showed that NALCN channels are closely related to fungal calcium channels, which they functionally resemble. Here, we use this relationship to predict a family of NALCN-associated proteins in animals on the basis of homology with the yeast protein Mid1, the subunit of the yeast calcium channel. These proteins all share a cysteine-rich region that is necessary for Mid1 function in yeast. We validate this predicted association by showing that the Mid1 homolog in Drosophila, encoded by the CG33988 gene, is coordinately expressed with NALCN, and that knockdown of either protein creates identical phenotypes in several behaviors associated with NALCN function. The relationship between Mid1 and leak channels has therefore persisted over a billion years of evolution, despite drastic changes to both proteins and the organisms in which they exist.
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影响因子：
14.9
作者：
Hunter S;Jones P;Mitchell A;Apweiler R;Attwood TK;Bateman A;Bernard T;Binns D;Bork P;Burge S;de Castro E;Coggill P;Corbett M;Das U;Daugherty L;Duquenne L;Finn RD;Fraser M;Gough J;Haft D;Hulo N;Kahn D;Kelly E;Letunic I;Lonsdale D;Lopez R;Madera M;Maslen J;McAnulla C;McDowall J;McMenamin C;Mi H;Mutowo-Muellenet P;Mulder N;Natale D;Orengo C;Pesseat S;Punta M;Quinn AF;Rivoire C;Sangrador-Vegas A;Selengut JD;Sigrist CJ;Scheremetjew M;Tate J;Thimmajanarthanan M;Thomas PD;Wu CH;Yeats C;Yong SY
通讯作者：
Yong SY
影响因子：
5.3
作者：
IIDA, H;NAKAMURA, H;ANRAKU, Y
通讯作者：
ANRAKU, Y
影响因子：
9.2
作者：
Humphrey, John A.;Hamming, Kevin S.;Nash, Howard A.
通讯作者：
Nash, Howard A.
肽神经递质激活 NALCN 和 UNC-80 的阳离子通道复合物。
影响因子：
64.8
作者：
Lu, Boxun;Su, Yanhua;Das, Sudipto;Wang, Haikun;Wang, Yan;Liu, Jin;Ren, Dejian
通讯作者：
Ren, Dejian
影响因子：
64.8
作者：
Graveley BR;Brooks AN;Carlson JW;Duff MO;Landolin JM;Yang L;Artieri CG;van Baren MJ;Boley N;Booth BW;Brown JB;Cherbas L;Davis CA;Dobin A;Li R;Lin W;Malone JH;Mattiuzzo NR;Miller D;Sturgill D;Tuch BB;Zaleski C;Zhang D;Blanchette M;Dudoit S;Eads B;Green RE;Hammonds A;Jiang L;Kapranov P;Langton L;Perrimon N;Sandler JE;Wan KH;Willingham A;Zhang Y;Zou Y;Andrews J;Bickel PJ;Brenner SE;Brent MR;Cherbas P;Gingeras TR;Hoskins RA;Kaufman TC;Oliver B;Celniker SE
通讯作者：
Celniker SE