Novel MEA platform with PDMS microtunnels enables the detection of action potential propagation from isolated axons in culture.
Novel MEA platform with PDMS microtunnels enables the detection of action potential propagation from isolated axons in culture.
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DOI：
10.1039/b806689b
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发表时间：
2009-02-07
期刊：
影响因子：
6.1
通讯作者：
Wheeler BC
中科院分区：
文献类型：
--
作者：
Dworak BJ;Wheeler BC
本研究研究了一种新的多电极阵列（MEA）设计，能够使用PDMS微隧道长期和高选择性地记录轴突信号。我们成功地在培养液中培养出神经元，使得只有轴突延伸通过狭窄（10 μ m宽，3 μ m高）和长（750 μ m）的微隧道，在微隧道下集成了多个电极。这允许记录相对较大（高达200µV）的电信号，包括这些移动动作电位的传播速度和方向。为了进一步演示该装置作为药物筛选分析的诊断工具的操作，在冲洗实验中应用了药物甲哌卡因。在这里，我们发现了平均尖峰率和传导速度的显著变化。
This study investigated a novel multi-electrode-array (MEA) design capable of long-term and highly selective recordings of axonal signals using PDMS microtunnels. We successfully grew neurons in culture so that only axons extended through narrow (10 µm wide by 3 µm high) and long (750 µm) microtunnels under which multiple electrodes were integrated. This permitted the recording of relatively large (up to 200 µV) electrical signals, including the propagation speed and direction of these travelling action potentials. To further demonstrate the operation of the device as a diagnostic tool for drug screening assays, the drug mepivacaine was applied in washout experiments. Here, we identified significant changes in mean spiking rate and conduction velocity.
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影响因子：
3.9
作者：
Taylor, AM;Rhee, SW;Jeon, NL
通讯作者：
Jeon, NL
影响因子：
4.6
作者：
Buitenweg, JR;Rutten, WLC;Marani, E
通讯作者：
Marani, E
影响因子：
2.5
作者：
Meeks, Julian P.;Mennerick, Steven
通讯作者：
Mennerick, Steven
影响因子：
3.4
作者：
Kondo, M;Iwase, S;Kuzuhara, S
通讯作者：
Kuzuhara, S
DOI：
10.1049/ip-nbt:20040706
发表时间：
2004-06-01
期刊：
IEE proceedings. Nanobiotechnology
影响因子：
--
作者：
Nam, Y;Chang, J;Wheeler, B C
通讯作者：
Wheeler, B C