Phosphatidic acid synthesis in bacteria.
Phosphatidic acid synthesis in bacteria.
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DOI：
10.1016/j.bbalip.2012.08.018
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发表时间：
2013-03
期刊：
影响因子：
--
通讯作者：
Rock CO
中科院分区：
文献类型：
--
作者：
Yao J;Rock CO
膜磷脂合成是细菌生理学的重要方面。虽然细菌产生的磷脂头基结构的谱很大，但所有这些分子的关键前体是磷脂酸（PtdOH）。通过甘油-磷酸合酶从糖酵解衍生的甘油-3-磷酸是PtdOH的甘油骨架的通用来源。有两个不同的酶家族负责甘油-3-磷酸1-位的酰化。PlsB酰基转移酶在大肠杆菌中发现，并且在许多真核生物中存在同源物。该蛋白质家族主要使用脂肪酸合成的酰基-酰基载体蛋白（ACP）终产物作为酰基供体，但也可以使用源自外源脂肪酸的酰基-CoA。第二个蛋白质家族PlsY在细菌中分布更广泛，并利用独特的酰基供体酰基磷酸盐，其由酶PlsX从酰基-ACP产生。2-位的酰化由PlsC蛋白家族的成员进行。所有的PlsC都使用酰基-ACP作为酰基供体，尽管γ-变形菌的PlsC也可以使用酰基-CoA。磷脂头基是其他膜相关分子生物合成的前体，这些反应的二酰基甘油产物通过两种不同的脂质激酶家族之一转化为PtdOH。PtdOH的从头和再循环途径在细胞生理学中的核心重要性表明这些酶是革兰氏阳性病原体中开发抗菌治疗剂的合适靶标。这篇文章是题为磷脂和磷脂代谢的特刊的一部分。
Membrane phospholipid synthesis is a vital facet of bacterial physiology. Although the spectrum of phospholipid headgroup structures produced by bacteria is large, the key precursor to all of these molecules is phosphatidic acid (PtdOH). Glycerol-3-phosphate derived from the glycolysis via glycerol-phosphate synthase is the universal source for the glycerol backbone of PtdOH. There are two distinct families of enzymes responsible for the acylation of the 1-position of glycerol-3-phosphate. The PlsB acyltransferase was discovered in Escherichia coli, and homologs are present in many eukaryotes. This protein family primarily uses acyl-acyl carrier protein (ACP) endproducts of fatty acid synthesis as acyl donors, but may also use acyl-CoA derived from exogenous fatty acids. The second protein family, PlsY, is more widely distributed in bacteria and utilizes the unique acyl donor, acyl-phosphate, which is produced from acyl-ACP by the enzyme PlsX. The acylation of the 2-position is carried out by members of the PlsC protein family. All PlsCs use acyl-ACP as the acyl donor, although the PlsCs of the γ-proteobacteria also may use acyl-CoA. Phospholipid headgroups are precursors in the biosynthesis of other membrane-associated molecules and the diacylglycerol product of these reactions is converted to PtdOH by one of two distinct families of lipid kinases. The central importance of the de novo and recycling pathways to PtdOH in cell physiology suggest these enzymes are suitable targets for the development of antibacterial therapeutics in Gram-positive pathogens. This article is part of a Special Issue entitled Phospholipids and Phospholipid Metabolism.
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DOI：
10.1007/bf00280009
发表时间：
1992-03-01
期刊：
MOLECULAR & GENERAL GENETICS
影响因子：
--
作者：
COLEMAN, J
通讯作者：
COLEMAN, J
影响因子：
2.6
作者：
CORRE, J;LUCCHINI, JJ;CREMIEUX, A
通讯作者：
CREMIEUX, A
DOI：
10.1099/00221287-144-2-449
发表时间：
1998-02-01
期刊：
MICROBIOLOGY-UK
影响因子：
--
作者：
Glatz, E;Persson, M;Rutberg, B
通讯作者：
Rutberg, B
影响因子：
3.2
作者：
CRONAN, JE;BELL, RM
通讯作者：
BELL, RM
影响因子：
2.9
作者：
Badger, J;Sauder, JM;Harris, TJR
通讯作者：
Harris, TJR