Effects of immune stress on performance parameters, intestinal enzyme activity and mRNA expression of intestinal transporters in broiler chickens.
Effects of immune stress on performance parameters, intestinal enzyme activity and mRNA expression of intestinal transporters in broiler chickens.
复制标题
免疫应激对肉鸡生产性能参数、肠道酶活性和肠道转运蛋白mRNA表达的影响
DOI：
10.5713/ajas.2011.11377
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发表时间：
2012-05
期刊：
影响因子：
--
通讯作者：
Yao JH
中科院分区：
文献类型：
--
作者：
Feng Y;Yang XJ;Wang YB;Li WL;Liu Y;Yin RQ;Yao JH
免疫应激是由外力引起的免疫稳态的丧失。本试验旨在研究免疫应激对肉鸡生长性能、小肠酶和蠕动率以及营养转运蛋白mRNA表达的影响。选取432只1日龄肉鸡（Cobb500），随机分为4组进行处理；每组包括9个笼子，每个笼子12只鸟。组1 =无疫苗（NV）；组2 =常规疫苗（CV）；3组=脂多糖(LPS)+常规疫苗（LPS）；第4组=环磷酰胺(CYP)+常规疫苗（CYP）。结果表明，LPS和CYP免疫应激降低了仔猪增重（BWG）、采食量（FI）、小肠蠕动率和小肠食糜sIgA含量（p<0.05）。饲料系数（FCR）在饲喂期内基本保持不变。LPS和CYP提高了肠道酶活性，增加了SGLT-1、CaBP-D28k和L-FABP mrna的相对表达量（p<0.05）。LPS和CYP注射液对健康肉鸡生长性能有负面影响。本研究表明，在可控的合理饲养环境下，NV和CV能提高生长性能，但降低小肠酶活性和营养转运蛋白mRNA的相对表达量。一般建议，肉鸡只需要接种它们可能接触到的疾病的疫苗。
Immune stress is the loss of immune homeostasis caused by external forces. The purpose of this experiment was to investigate the effects of immune stress on the growth performance, small intestinal enzymes and peristalsis rate, and mRNA expression of nutrient transporters in broiler chickens. Four hundred and thirty-two 1-d-old broilers (Cobb500) were randomly assigned to four groups for treatment; each group included nine cages with 12 birds per cage. Group 1 = no vaccine (NV); Group 2 = conventional vaccine (CV); group 3 = lipopolysaccharide (LPS)+conventional vaccine (LPS); group 4 = cyclophosphamide (CYP)+conventional vaccine (CYP). The results demonstrated that immune stress by LPS and CYP reduced body weight gain (BWG), feed intake (FI), small intestine peristalsis rate and sIgA content in small intestinal digesta (p<0.05). However, the feed conversion ratio (FCR) remained unchanged during the feeding period. LPS and CYP increased intestinal enzyme activity, relative expression of SGLT-1, CaBP-D28k and L-FABP mRNAs (p<0.05). LPS and CYP injection had a negative effect on the growth performance of healthy broiler chickens. The present study demonstrated that NV and CV could improve growth performance while enzyme activity in small intestine and relative expression of nutrient transporter mRNA of NV and CV were decreased in the conditions of a controlled rational feeding environment. It is generally recommended that broilers only need to be vaccinated for the diseases to which they might be exposed.
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影响因子：
1.9
作者：
PROWS, DR;MURPHY, EJ;SCHROEDER, F
通讯作者：
SCHROEDER, F
DOI：
10.1152/ajpregu.00393.2005
发表时间：
2006-01-01
影响因子：
2.8
作者：
Garriga, C;Hunter, RR;Moretó, M
通讯作者：
Moretó, M
DOI：
10.1016/j.cbpc.2004.09.013
发表时间：
2004-12-01
影响因子：
2.2
作者：
Lin, H;Decuypere, E;Buyse, J
通讯作者：
Buyse, J
影响因子：
2.9
作者：
SAVORY, CJ
通讯作者：
SAVORY, CJ
影响因子：
2
作者：
LESSIRE, M
通讯作者：
LESSIRE, M