Biomimetic behaviors in hydrogel artificial cells through embedded organelles.
Biomimetic behaviors in hydrogel artificial cells through embedded organelles.
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水凝胶人造细胞中的仿生行为通过嵌入的细胞器。
DOI：
10.1073/pnas.2307772120
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发表时间：
2023-08-29
影响因子：
11.1
通讯作者：
Elani, Yuval
中科院分区：
文献类型：
--
作者：
Allen, Matthew E.;Hindley, James W.;O'Toole, Nina;Cooke, Hannah S.;Contini, Claudia;Law, Robert, V;Ces, Oscar;Elani, Yuval
细胞的形式和功能可以通过被称为人造细胞的实体来模仿。人工细胞的一个新兴底盘是水凝胶微粒，由于其复制细胞内存在的凝胶样环境的能力，并且更广泛地说，由于其生物技术上有用的特性，水凝胶微粒正变得越来越突出。然而，有有限的策略来创建复杂的基于水凝胶的人工细胞。在这里，我们展示了一种微流体生产策略，通过将一系列模块化的，可互换的亚室到水凝胶微粒的水凝胶人工细胞。然后，我们利用这个亚隔室工具包来生产具有一系列相关生物学行为的水凝胶人工细胞，包括运动，传感和通信。这为生产可用于一系列生物技术应用的更复杂的水凝胶人工细胞铺平了道路。人工细胞是由分子构建块形成的仿生结构，其复制生物过程、行为和架构。在这些结构单元中，水凝胶已经成为理想的，但未充分利用的候选物，以提供凝胶状底盘，其中掺入生物和非生物组分，从而能够复制细胞功能。在这里，我们展示了一种微流体策略，组装生物相容性细胞大小的基于水凝胶的人工细胞与各种不同的嵌入式功能亚室，作为工程合成细胞器。这些细胞器使得能够重建越来越多的仿生行为，包括刺激诱导的运动，通过激活膜相关蛋白质的内容物释放，以及与周围生物灵感隔室的酶促通信。通过这种方式，我们展示了自下而上构建基于水凝胶的人工细胞微系统的基本策略，该系统复制了基本的细胞行为，为构建下一代生物技术设备铺平了道路。
Cellular form and functionality can be mimicked through entities known as artificial cells. One emerging chassis for artificial cells are hydrogel microparticles, which are gaining prominence due to their ability to replicate the gel-like environments present within cells, and more broadly, because of their biotechnologically useful properties. However, there are limited strategies to create sophisticated hydrogel-based artificial cells. Here we demonstrate a microfluidic production strategy for hydrogel artificial cells by incorporating a range of modular, interchangeable subcompartments into hydrogel microparticles. We then leverage this subcompartment toolkit to produce hydrogel artificial cells with a range of relevant biological behaviors including motility, sensing, and communication. This paves the way to the production of more sophisticated hydrogel artificial cells that can be used for a range of biotechnological applications. Artificial cells are biomimetic structures formed from molecular building blocks that replicate biological processes, behaviors, and architectures. Of these building blocks, hydrogels have emerged as ideal, yet underutilized candidates to provide a gel-like chassis in which to incorporate both biological and nonbiological componentry which enables the replication of cellular functionality. Here, we demonstrate a microfluidic strategy to assemble biocompatible cell-sized hydrogel-based artificial cells with a variety of different embedded functional subcompartments, which act as engineered synthetic organelles. The organelles enable the recreation of increasingly biomimetic behaviors, including stimulus-induced motility, content release through activation of membrane-associated proteins, and enzymatic communication with surrounding bioinspired compartments. In this way, we showcase a foundational strategy for the bottom–up construction of hydrogel-based artificial cell microsystems which replicate fundamental cellular behaviors, paving the way for the construction of next-generation biotechnological devices.
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影响因子：
4.6
作者：
Elani Y;Trantidou T;Wylie D;Dekker L;Polizzi K;Law RV;Ces O
通讯作者：
Ces O
影响因子：
7.4
作者：
Friddin, Mark S.;Elani, Yuval;Ces, Oscar
通讯作者：
Ces, Oscar
聚合细胞模拟中的光激活膜转运。
影响因子：
16.6
作者：
Cao, Shoupeng;da Silva, Lucas Caire;Landfester, Katharina
通讯作者：
Landfester, Katharina
DOI：
10.1073/pnas.2206563119
发表时间：
2022-10-18
影响因子：
11.1
作者：
Gispert, Ignacio;Hindley, James W.;Pilkington, Colin P.;Shree, Hansa;Barter, Laura M. C.;Ces, Oscar;Elani, Yuval;Weitz, David
通讯作者：
Weitz, David
影响因子：
3.2
作者：
Clayton, Katherine N.;Salameh, Janelle W.;Kinzer-Ursem, Tamara L.
通讯作者：
Kinzer-Ursem, Tamara L.