Dysbiosis of Fecal Microbiota From Complement 3 Knockout Mice With Constipation Phenotypes Contributes to Development of Defecation Delay.
Dysbiosis of Fecal Microbiota From Complement 3 Knockout Mice With Constipation Phenotypes Contributes to Development of Defecation Delay.
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DOI：
10.3389/fphys.2021.650789
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发表时间：
2021
影响因子：
4
通讯作者：
Hwang DY
中科院分区：
文献类型：
--
作者：
Choi YJ;Kim JE;Lee SJ;Gong JE;Son HJ;Hong JT;Hwang DY
在补体 3 (C3) 敲除 (KO) 小鼠中检测到显着的便秘表型，但尚未进行与肠道微生物群改变相关的研究。为了研究菌群失调对便秘 C3 KO 小鼠粪便微生物群的影响，对 16 周龄 C3 KO 小鼠中结肠中粪便微生物群的组成进行了表征，并在 C3 KO 小鼠粪便微生物群移植（FMT）后检查了它们对排便延迟发育的功能。与野生型 (WT) 小鼠相比，16 周龄 C3 KO 小鼠的便秘表型（包括粪便参数和组织病理学结构）发生了一些显着变化。 C3 KO 小鼠的粪便微生物群显示厌氧柱菌、棒状杆菌、克里斯滕森氏菌、动丝菌和振荡杆菌种群减少，而普雷沃氏菌属、罗伊氏杆菌、普雷沃氏菌、真杆菌、文化菌、拟杆菌和穆里杆菌种群增加。在 FMT 研究中，抗生素诱导的微生物群耗竭 (AiDM)-WT 和 AiDM-KO 小鼠移植 KO (KFMT) 组的关键粪便参数，包括重量和含水量显着下降，并且粪便形态也观察到类似的变化。然而，与 AiDM-KO 小鼠相比，仅 KFMT 组 AiDM-WT 小鼠的肠长度有所缩短。 AiDM-WT 和 AiDM-KO 小鼠 KFMT 组的粘膜层和肌肉厚度普遍减少，并且在同一组中观察到 Lieberkuhn 隐窝结构和分子调节因子（包括 AQP8、C-kit 和 5-HT）的显着改变。综上所述，本研究结果表明，具有便秘表型的 C3 KO 小鼠粪便微生物群失调在诱导和调节排便延迟中发挥着关键作用。
Significant phenotypes for constipation were detected in complement 3 (C3) knockout (KO) mice, although no research has been conducted on an association with alteration of gut microbiota. To investigate the effects of dysbiosis on fecal microbiota from C3 KO mice with constipation, the composition of fecal microbiota was characterized in mid-colons of 16-week-old C3 KO mice, and their function for defecation delay development was examined after fecal microbiota transplantation (FMT) of C3 KO mice. Some significant alterations in constipation phenotypes, including stool parameters and histopathological structure, were detected in 16-week-old C3 KO mice compared to those of wild-type (WT) mice. Fecal microbiota of C3 KO mice exhibited decreases in Anaerocolumna, Caecibacterium, Christensenella, Kineothrix, and Oscillibacter populations and increases in Prevotellamassilia, Reuthenibacterium, Prevotella, Eubacterium, Culturomica, Bacteroides, and Muribaculum populations. In FMT study, key stool parameters, including weight and water content, were remarkably declined in a transplanted KO (KFMT) group of antibiotics-induced depletion of microbiota (AiDM)-WT and AiDM-KO mice, and a similar change was observed in fecal morphology. However, intestine length decreased in only the KFMT group of AiDM-WT mice compared with that of AiDM-KO mice. The mucosal layer and muscle thickness were commonly decreased in the KFMT group of AiDM-WT and AiDM-KO mice, and significant alterations in the crypt structure of Lieberkuhn and molecular regulators, including AQP8, C-kit, and 5-HT, were observed in the same group. Taken together, results of the present study indicate that dysbiosis of fecal microbiota from C3 KO mice with constipation phenotypes has a key role in the induction and regulation of defecation delay.
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影响因子：
10.9
作者：
Hand, Timothy W.;Vujkovic-Cvijin, Ivan;Ridaura, Vanessa K.;Belkaid, Yasmine
通讯作者：
Belkaid, Yasmine
DOI：
10.1111/1440-1681.12792
发表时间：
2017-10-01
影响因子：
2.9
作者：
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通讯作者：
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影响因子：
5.6
作者：
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通讯作者：
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影响因子：
3.5
作者：
Gerritsen, Jacoline;Smidt, Hauke;Rijkers, Ger T.;de Vos, Willem M.
通讯作者：
de Vos, Willem M.
影响因子：
4.6
作者：
Cao H;Liu X;An Y;Zhou G;Liu Y;Xu M;Dong W;Wang S;Yan F;Jiang K;Wang B
通讯作者：
Wang B