A dispersive solid phase extraction adsorbent based on aptamer modified chitosan nanofibers for zearalenone separation in corn, wheat, and beer samples.
A dispersive solid phase extraction adsorbent based on aptamer modified chitosan nanofibers for zearalenone separation in corn, wheat, and beer samples.
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一种基于适配体改性壳聚糖纳米纤维的分散固相萃取吸附剂，用于玉米、小麦和啤酒样品中的玉米赤霉烯酮分离。
DOI：
10.1039/d0ay01637c
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发表时间：
2020-11
期刊：
影响因子：
--
通讯作者：
Xiaogang Hu
中科院分区：
文献类型：
--
作者：
Luying Liu;Yanxia Ma;Xiaoting Zhang;Xixiang Yang;Xiaogang Hu
食品中痕量生物毒素的高选择性分离一直是分析化学家们研究的热点。本文以冷冻干燥法制备的壳聚糖纳米纤维为载体，采用核酸适体对纳米纤维进行修饰，用于分散固相萃取（dSPE）分析痕量玉米赤霉烯酮。壳聚糖纳米纤维的形貌均匀连续，长度为微米级，直径约为400 nm。由于壳聚糖纳米纤维具有较高的比表面积，在5 mg壳聚糖纳米纤维上的固定量高达10.1 μg，且具有良好的稳定性和重复性。适体修饰的壳聚糖纳米纤维（Apt-CNFs）显示出特定的选择性玉米赤霉烯酮的选择性系数为2.65相比，乱序寡核苷酸功能化的CNFs，和其他类似物和参考化合物的选择性因子为1.57至50.0。在优化提取条件后，将Apt-CNF dSPE与高效液相色谱联用检测玉米赤霉烯酮，线性范围为0.06-10.0 μg L-1，检出限为18.0 ng L-1。玉米、小麦和啤酒样品中玉米赤霉烯酮的加标回收率分别为101- 108%、100- 110%和98.3-101%。玉米和小麦中玉米赤霉烯酮的残留量分别为0.365和0.0775 μg g-1。
Highly selective separation of trace bio-toxins in food samples has long been a hot topic pursued by analytical chemists. In this paper, chitosan nanofibers prepared by freeze-drying were modified with aptamers for dispersive solid phase extraction (dSPE) of trace zearalenone. The morphology of achieved chitosan nanofibers was found to be uniform and continuous, and the length was at the micron level with about a 400 nm diameter. The immobilization capacity of the aptamer was as high as 10.1 μg on 5 mg chitosan nanofibers with good stability and repeatability, owing to the high specific surface area of nanofibers. The aptamer modified chitosan nanofibers (Apt-CNFs) showed specific selectivity to zearalenone with a selectivity coefficient of 2.65 compared to the scrambled oligonucleotide functionalized CNFs, and the selectivity factors over other analogs and reference compounds were from 1.57 to 50.0. After the optimization of extraction conditions, the Apt-CNF based dSPE was coupled with high-performance liquid chromatography for zearalenone monitoring, and a good linear range of 0.06-10.0 μg L-1 was achieved with a detection limit of 18.0 ng L-1. The spiking recoveries of 101-108%, 100-110%, and 98.3-101% were achieved for trace zearalenone in corn, wheat, and beer samples, respectively. The residual zearalenone was detected in corn and wheat with a content of 0.365 and 0.0775 μg g-1, respectively.
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影响因子：
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