Simplification of the modified Gallyas method.
Simplification of the modified Gallyas method.
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DOI：
10.1111/neup.12144
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发表时间：
2015-02
期刊：
影响因子：
--
通讯作者：
Saito Y
中科院分区：
文献类型：
--
作者：
Kuninaka N;Kawaguchi M;Ogawa M;Sato A;Arima K;Murayama S;Saito Y
Galyas方法是一种在神经病理学领域中必不可少的银浸渍技术，因为它对于检测中枢神经系统中的嗜银包涵体具有很高的灵敏度。在日本，加里亚斯法得到了改进，被广泛应用为“改进的加里亚斯法”。然而，由于需要特殊的试剂、几种染色工艺和熟练的技术，这种方法在普通病理实验室中并不普遍使用。本研究的目的是提供一种简化的Galyas方法。我们省略了染色过程中的硝酸镧步骤，并与原始方法以及免疫组织化学相比较，验证了该方法的充分性，使用了阿尔茨海默病、嗜银颗粒病、多系统萎缩、Pick病和路易体病患者的标本。与改良的Galyas法相比，简化的方法对档案组织中的所有结构都有很好的染色效果，染色时间明显缩短。改进的Galyas方法可以省去硝酸镧步骤，从而减少了所需试剂的数量，缩短了染色时间。
The Gallyas method is a silver impregnation technique that is essential in the field of neuropathology because of its high sensitivity for the detection of argentophilic inclusion bodies in the central nervous system. In Japan, the Gallyas method has improved and is widely used as the “modified Gallyas method”. However, this method is not popularly used in general pathology laboratories because of the need for special reagents, several staining processes, and skilled techniques. The objective of the current study was to provide a simplified Gallyas method. We omitted the lanthanum nitrate step from the staining process and verified the adequacy in comparison with the original method as well as immunohistochemistry, using specimens from patients of Alzheimer's disease, argyrophilic grain disease, multiple system atrophy, Pick's disease, and Lewy body disease. The simplified method provided good staining to all the structures in archival tissues, compared with the modified Gallyas method in a significantly shorter staining time. The lanthanum nitrate step can be omitted from the modified Gallyas method, resulting in reduction in the number of reagents required and shortening of the staining time.
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影响因子：
2.3
作者：
Arai, N;Oda, M
通讯作者：
Oda, M
影响因子：
--
作者：
BRAAK, H;BRAAK, E;BOHL, J
通讯作者：
BOHL, J
DOI：
10.1002/path.1700240109
发表时间：
1921-01-01
期刊：
JOURNAL OF PATHOLOGY AND BACTERIOLOGY
影响因子：
--
作者：
Perdrau, JR
通讯作者：
Perdrau, JR
影响因子：
3.2
作者：
LILLIE, RD
通讯作者：
LILLIE, RD
影响因子：
12.7
作者：
Uchihara, T;Nakamura, A;Mizutani, T
通讯作者：
Mizutani, T