Ferritin enhances SPIO tracking of C6 rat glioma cells by MRI.
Ferritin enhances SPIO tracking of C6 rat glioma cells by MRI.
复制标题
铁蛋白增强 MRI 对 C6 大鼠胶质瘤细胞的 SPIO 追踪
DOI：
10.1007/s11307-010-0338-5
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发表时间：
2011-02
影响因子：
3.1
通讯作者：
Chen, Xiaoyuan
中科院分区：
文献类型：
--
作者：
Wang, Jiandong;Xie, Jin;Zhou, Xiaojun;Cheng, Zhen;Gu, Ning;Teng, Gaojun;Hu, Qiujue;Zhu, Feipeng;Chang, Shuanghui;Zhang, Fan;Lu, Guangming;Chen, Xiaoyuan
研究铁蛋白过表达对C6大鼠胶质瘤细胞超顺磁性氧化铁（SPIO）颗粒标记的影响，并利用磁共振成像（MRI）在体内追踪标记细胞。
构建小鼠铁蛋白基因H链的质粒并转染到C6细胞中。将亲代C6细胞以及转染后的C6细胞用SPIO标记后，双侧皮下接种到裸鼠体内。在接种C6细胞后，对小鼠进行多次T2加权磁共振扫描成像。2周后处死小鼠，通过电感耦合等离子体测量肿瘤组织中的铁浓度。
转染铁蛋白质粒且用SPIO标记的C6细胞所形成的异种移植物中的铁浓度显著高于未转染但用SPIO标记的亲代C6细胞所形成的异种移植物（p = 0.034，N = 5）。与未转染但用SPIO标记的亲代细胞相比，转染铁蛋白的C6细胞在体内显示出更好的T2对比度。
铁蛋白与SPIO协同可使MRI细胞追踪时间更长。
To investigate the effect of ferritin protein overexpression on superparamagnetic iron oxide (SPIO) particle labeling of C6 rat glioma cells, and track the labeled cells in vivo using magnetic resonance imaging (MRI). A plasmid of H-chain of murine ferritin gene was constructed and transfected into C6 cells. The parental and the transfected C6 cells labeled with SPIO were bilaterally inoculated subcutaneously into nude mice. The mice were imaged by multiple T2-weighted MR scans after C6 cell inoculation. The mice were killed 2 weeks later, and the concentration of iron in the tumor tissue was measured by inductively coupled plasma. The iron concentration in xenografts derived from SPIO-labeled C6 cells that were transfected with ferritin plasmid was significantly higher than that in xenografts from parental C6 cells that were labeled with SPIO but not transfected (p=0.034, N=5). Ferritin-transfected C6 cells showed an improved T2 contrast in vivo compared with parental cells labeled with SPIO but not transfected. Coordinating ferritin with SPIO can lead to a longer MRI cellular tracking period.
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影响因子：
2.9
作者：
Pawelczyk, Edyta;Arbab, Ali S.;Frank, Joseph A.
通讯作者：
Frank, Joseph A.
DOI：
10.1016/j.bbrc.2008.02.159
发表时间：
2008-05-16
影响因子：
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作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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