Electrospun poly(lactic acid-co-glycolic acid) scaffolds for skin tissue engineering.
Electrospun poly(lactic acid-co-glycolic acid) scaffolds for skin tissue engineering.
复制标题
用于皮肤组织工程的电纺聚多（乳酸 - 乙醇酸）支架。
DOI：
10.1016/j.biomaterials.2008.06.028
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发表时间：
2008-10
期刊：
影响因子：
14
通讯作者：
Laurencin, Cato T.
中科院分区：
文献类型：
--
作者：
Kumbar, Sangamesh G.;Nukavarapu, Syam P.;James, Roshan;Nair, Lakshmi S.;Laurencin, Cato T.
研究了由不同纤维直径的支架组成的电纺纤维基质在严重皮肤缺损中的潜在应用。很少有系统的研究已经进行，以检查不同的纤维直径的电纺纤维基质的皮肤再生的效果。本研究报道了通过静电纺丝制备纤维直径为150 - 225、200 - 300、250 - 467、500 - 900、600 - 1200、2500 - 3000和3250 - 6000 nm的聚[乳酸-共-乙醇酸]（PLAGA）基质。发现所有纤维基质具有39.23 ± 8.15至79.21 ± 13.71 MPa的拉伸模量，其福尔斯落在正常人皮肤的范围内。此外，多孔纤维基质具有38 - 60%的孔隙率和10 - 14 μ m的平均孔径。我们评估了这些可生物降解的纤维基质作为皮肤替代品的功效，通过将它们与人皮肤成纤维细胞（hSF）接种。人皮肤成纤维细胞获得了良好的扩散形态，并在350 - 1100 nm直径范围内的纤维基质上显示出显著的进行性生长。在纤维直径为350 - 1100 nm的基质上接种的hSF中，III型胶原基因表达显著上调。根据需要，所提出的纤维皮肤替代物可以成功地制造和优化皮肤成纤维细胞附着和生长。
Electrospun fiber matrices composed of scaffolds of varying fiber diameters were investigated for potential application of severe skin loss. Few systematic studies have been performed to examine the effect of varying fiber diameter electrospun fiber matrices for skin regeneration. The present study reports the fabrication of poly[lactic acid-co-glycolic acid] (PLAGA) matrices with fiber diameters of 150–225, 200–300, 250–467, 500–900, 600–1200, 2500–3000 and 3250–6000 nm via electrospinning. All fiber matrices found to have a tensile modulus from 39.23 ± 8.15 to 79.21 ± 13.71 MPa which falls in the range for normal human skin. Further, the porous fiber matrices have porosity between 38–60 % and average pore diameters between 10–14µm. We evaluated the efficacy of these biodegradable fiber matrices as skin substitutes by seeding them with human skin fibroblasts (hSF). Human skin fibroblasts acquired a well spread morphology and showed significant progressive growth on fiber matrices in the 350–1100 nm diameter range. Collagen type III gene expression was significantly up-regulated in hSF seeded on matrices with fiber diameters in the range of 350–1100 nm. Based on the need, the proposed fiber skin substitutes can be successfully fabricated and optimized for skin fibroblast attachment and growth.
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影响因子：
9
作者：
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通讯作者：
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