The SFP1 gene product of Saccharomyces cerevisiae regulates G2/M transitions during the mitotic cell cycle and DNA-damage response.
The SFP1 gene product of Saccharomyces cerevisiae regulates G2/M transitions during the mitotic cell cycle and DNA-damage response.
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酿酒酵母的 SFP1 基因产物在有丝分裂细胞周期和 DNA 损伤反应期间调节 G2/M 转变。
DOI：
10.1093/genetics/150.4.1419
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发表时间：
1998
期刊：
影响因子：
3.3
通讯作者：
Norris,D
中科院分区：
文献类型：
--
作者：
Xu,Z;Norris,D
在真核细胞中，如果一个特定的事件未能适当地完成，或者如果一个重要的细胞内结构有缺陷或受损，检查点途径就会阻止细胞周期的进展。缺乏SFP 1基因的酿酒酵母菌株不能在G2 DNA损伤检查点响应基因组损伤而停止，但保持其在复制和纺锤体组装检查点停止的能力。Sfp 1 Δ突变体的特征在于在正常（未受损）细胞周期期间过早进入有丝分裂，而过表达Sfp 1 p的菌株在G2中表现出延迟。因此，Sfp 1 p在正常细胞周期和G2检查点通路中均充当G2/M转换的阻遏物。Sfp 1是一种核蛋白，具有两个Cys 2 His 2锌指结构域，通常在转录因子中发现。我们建议，Sfp 1 p调节基因产物的表达，参与G2/M转换在有丝分裂细胞周期和DNA损伤反应。为了支持这一模型，Sfp 1 p的过表达诱导了PDS 1基因的表达，该基因已知编码一种调节G2检查点的蛋白质。
In eukaryotic cells, checkpoint pathways arrest cell-cycle progression if a particular event has failed to complete appropriately or if an important intracellular structure is defective or damaged. Saccharomyces cerevisiae strains that lack the SFP1 gene fail to arrest at the G2 DNA-damage checkpoint in response to genomic injury, but maintain their ability to arrest at the replication and spindle-assembly checkpoints. sfp1Δ mutants are characterized by a premature entrance into mitosis during a normal (undamaged) cell cycle, while strains that overexpress Sfp1p exhibit delays in G2. Sfp1p therefore acts as a repressor of the G2/M transition, both in the normal cell cycle and in the G2 checkpoint pathway. Sfp1 is a nuclear protein with two Cys2His2zinc-finger domains commonly found in transcription factors. We propose that Sfp1p regulates the expression of gene products involved in the G2/M transition during the mitotic cell cycle and the DNA-damage response. In support of this model, overexpression of Sfp1p induces the expression of the PDS1 gene, which is known to encode a protein that regulates the G2 checkpoint.
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DOI：
10.1083/jcb.134.4.963
发表时间：
1996-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Jin P;Gu Y;Morgan DO
通讯作者：
Morgan DO
DOI：
10.1099/00221287-113-2-369
发表时间：
1979-01-01
期刊：
JOURNAL OF GENERAL MICROBIOLOGY
影响因子：
--
作者：
HUTTER, KJ;EIPEL, HE
通讯作者：
EIPEL, HE
影响因子：
10.5
作者：
CohenFix, O;Peters, JM;Koshland, D
通讯作者：
Koshland, D
影响因子：
64.5
作者：
ZHOU, Z;ELLEDGE, SJ
通讯作者：
ELLEDGE, SJ
通过 Northern 杂交和狭缝印迹杂交分析 RNA
影响因子：
--
作者：
T. Brown;Karol Mackey
通讯作者：
Karol Mackey