Nanofibrous Tubular Three-Dimensional Platform for Single Dental Pulp Stem Cell Polarization.
Nanofibrous Tubular Three-Dimensional Platform for Single Dental Pulp Stem Cell Polarization.
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单牙髓干细胞极化纳米纤维管三维平台。
DOI：
10.1021/acsami.0c17730
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发表时间：
2020-12-09
影响因子：
9.5
通讯作者：
Liu X
中科院分区：
文献类型：
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作者：
Chang B;Ma C;Liu X
牙髓干细胞（DPSCs）是再生牙髓学的主要干细胞来源。DPSCs需要经历一个极化过程并保持永久的极化状态才能发挥成牙髓细胞的功能。然而，控制DPSC极化的因素及其潜在机制尚不清楚。在这项研究中，我们建立了一个独特的纳米纤维管三维（3D）平台来探索DPSC极化。3D平台具有“干净”的背景，并且在平台的每个微岛中只限制一个DPSC；因此，它能够破译任何启动或调节DPSC极化的信号。利用仿生平台，我们发现纳米纤维管结构是引发DPSC极化的关键因素。动态形态学观察表明，极化后的DPSCs的细胞过程不断延长，并在72 h时达到平台期。同时，细胞极化标记物高尔基体从核旁区域不断移动，穿过细胞核，最终在距离细胞核几微米的位置稳定下来。抑制微丝和微管聚合证明了细胞骨架重组在调节DPSC极化中不可或缺的作用。此外，细胞张力参与了DPSC极化的调控。这项工作的发现扩大了对DPSC极化的深入了解，有助于设计新的再生牙髓学生物启发材料。
Dental pulp stem cells (DPSCs) are the primary stem cell source for regenerative endodontics. DPSCs need to undergo a polarization process and retain the permanent polarization status to perform the function of odontoblasts. However, the factors that control DPSC polarization and its underlying mechanism remain unknown. In this study, we established a unique nanofibrous tubular three-dimensional (3D) platform to explore DPSC polarization. The 3D platform has a “clean” background and confines one single DPSC in each microisland of the platform; therefore, it is capable of deciphering any signal that initiates or regulates DPSC polarization. Using the biomimetic platform, we identified that the nanofibrous tubular architecture is the crucial factor to initiate DPSC polarization. Dynamic morphological observation showed that the cellular process of the polarized DPSCs continuously extended and reached a plateau at 72 h. Meanwhile, Golgi apparatus, a cell polarization marker, continuously moved from a juxtanuclear region, passed the nucleus, and eventually settled down at a final position that was a few micrometers away from the nucleus. Inhibition of microfilament and microtubule polymerization demonstrated the indispensable role of cytoskeleton reorganization in modulating DPSC polarization. In addition, cell tension was involved in the regulation of DPSC polarization. The findings of this work expand the in-depth understanding of DPSC polarization, which helps design new bioinspired materials for regenerative endodontics.
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用于骨组织工程的仿生纳米纤维明胶/磷灰石复合支架。
影响因子：
14
作者：
Liu, Xiaohua;Smith, Laura A.;Hu, Jiang;Ma, Peter X.
通讯作者：
Ma, Peter X.
通过调节仿生基质的硬度来完成牙髓牙本质复合物的再生。
影响因子：
9.7
作者：
Qu, Tiejun;Jing, Junjun;Liu, Xiaohua
通讯作者：
Liu, Xiaohua
影响因子：
6.6
作者：
Chang, Bei;Svoboda, Kathy K. H.;Liu, Xiaohua
通讯作者：
Liu, Xiaohua
影响因子：
3
作者：
Li, Yucheng;Lu, Xin;Shi, Junnan
通讯作者：
Shi, Junnan
DOI：
10.1038/nrm2786
发表时间：
2009-11
期刊：
Nature reviews. Molecular cell biology
影响因子：
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作者：
通讯作者：
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