The effect of Lycium barbarum on spinal cord injury, particularly its relationship with M1 and M2 macrophage in rats.
The effect of Lycium barbarum on spinal cord injury, particularly its relationship with M1 and M2 macrophage in rats.
复制标题
DOI：
10.1186/1472-6882-13-67
复制
发表时间：
2013-03-22
影响因子：
--
通讯作者：
Ju G
中科院分区：
文献类型：
--
作者：
Zhang YK;Wang J;Liu L;Chang RC;So KF;Ju G
我们过去的研究表明L.枸杞子对中枢神经系统具有直接的神经保护和免疫调节作用，这与脊髓损伤事件密切相关，但尚未被研究。免疫反应在继发性损伤后的病理发展中起重要作用，特别是M1和M2型巨噬细胞，在本研究中特别强调。在我们以前的研究中，L。从损伤前7天开始口服施用枸杞以确保血液中的稳定浓度。临床上，L.只有在受伤后才能服用枸杞。因此，比较了损伤前和损伤后给药方案。进行体内和体外研究并进行化学分析，包括Western印迹。治疗前组的病灶大小明显大于治疗后组。为了解释这种差异，我们首先研究了L。枸杞作用于星形胶质细胞，形成环绕病变的胶质瘢痕。L.枸杞对星形胶质细胞无明显影响。研究了L. barbarum对小胶质细胞/巨噬细胞，特别是M1和M2极化的影响。脊髓损伤后，有害的M1细胞占主导地位的早期，而有益的M2细胞占主导地位的后期。我们发现，在预处理组L。枸杞多糖处理后，M2细胞活性明显增强，而枸杞多糖处理后，M1细胞活性明显增强，M2细胞活性明显降低。这解释了给药前和给药后组之间的差异。枸杞已被广泛接受，对各种中枢神经系统疾病有有益的作用。我们发现LBP在脊髓损伤早期给药的有害作用，提示应避免其应用。LBP在后期给药时的实质性有益效果对临床应用具有重要影响。
Our past researches suggested that L. barbarum exhibits direct neuroprotective and immune regulatory effects on the central nervous system, which are highly related to the events involved in the spinal cord injury, but not yet been investigated. Immune responses play an important role in the development of the pathology after secondary injury, particularly the M1 and M2 types of macrophage, on which special emphasis was laid in this study. In our previous studies L. barbarum was administrated orally from 7 days before the injury to ensure a stabilized concentration in the blood. For clinical application, L. barbarum can only be administered after the injury. Therefore, both pre-injury and post-injury administration protocols were compared. In vivo and in vitro studies were conducted and analyzed immunohistochemically, including Western blotting. The lesion size in the pre-treated group was much larger than that in the post-treated group. To explain this difference, we first studied the effect of L. barbarum on astrocytes, which forms the glial scar encircling the lesion. L. barbarum did not significantly affect the astrocytes. Then we studied the effect of L. barbarum on microglia/macrophages, particularly the M1 and M2 polarization. After spinal cord injury, the deleterious M1 cells dominant the early period, whereas the beneficial M2 cells dominate later. We found that in the pre-treated group L. barbarum significantly enhanced the expression of M1 cells and suppressed that of M2 cells, while in the post-treated group LBP markedly promoted the activity of M2 cells. This explained the difference between the pre- and post-treated groups. Lycium barbarum has been wildly accepted to have beneficial effects in various central nervous system diseases. Our finding of deleterious effect of LBP administered at early period of spinal cord injury, indicates that its application should be avoided. The substantial beneficial effect of LBP when administered at later stage has an important impact for clinical application.
登录
查看更多内容
DOI：
10.1523/jneurosci.3992-08.2009
发表时间：
2009-03-25
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Gensel JC;Nakamura S;Guan Z;van Rooijen N;Ankeny DP;Popovich PG
通讯作者：
Popovich PG
DOI：
10.1038/nri3073
发表时间：
2011-10-14
期刊：
Nature reviews. Immunology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1523/jneurosci.3257-09.2009
发表时间：
2009-10-28
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Kigerl KA;Gensel JC;Ankeny DP;Alexander JK;Donnelly DJ;Popovich PG
通讯作者：
Popovich PG
DOI：
10.1016/j.nurt.2010.07.002
发表时间：
2010-10
期刊：
Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics
影响因子：
--
作者：
Loane DJ;Byrnes KR
通讯作者：
Byrnes KR
影响因子：
4
作者：
Chiu, Kin;Zhou, Yuan;Chiu, Jin-Fu
通讯作者：
Chiu, Jin-Fu