Near-infrared micro-Raman spectroscopy for in vitro detection of cervical cancer.
Near-infrared micro-Raman spectroscopy for in vitro detection of cervical cancer.
复制标题
用于体外检测宫颈癌的近红外微拉曼光谱。
DOI：
10.1366/000370210790918364
复制
发表时间：
2010-03
影响因子：
3.5
通讯作者：
Acosta, Tayro
中科院分区：
文献类型：
--
作者：
Kamemoto, Lori E.;Misra, Anupam K.;Sharma, Shiv K.;Goodman, Marc T.;Luk, Hugh;Dykes, Ava C.;Acosta, Tayro
近红外拉曼光谱是一种强大的分析工具，用于检测生物样品中的关键差异，同时样品的天然荧光对拉曼光谱的干扰最小。该技术通常被认为是一种潜在的癌症筛查工具。在这篇文章中，我们报告在体外拉曼光谱的正常和癌变的人宫颈组织从7例，具有良好的信号噪声比，并被发现是可重复的。这些初步结果表明，这些光谱中的几个拉曼特征可用于区分癌性宫颈鳞状细胞与正常宫颈鳞状细胞。通常，宫颈癌细胞的拉曼光谱与正常鳞状细胞光谱相比显示强度差异。例如，在正常鳞状细胞的情况下，在775至975 cm-1区域中观察到几个明确的胶原拉曼峰，但在浸润性宫颈癌细胞的光谱中，这些峰低于正常拉曼光谱的检测限。在高频2800 ~ 3100 cm-1区域中，发现宫颈癌细胞的光谱中CH伸缩带下的峰面积与正常细胞的光谱相比低约6倍。由775 ~ 975 cm-1和2800 ~ 3100 cm-1区域的光谱特征得到的宫颈上皮中的癌细胞和正常细胞区域的拉曼化学图也显示出彼此良好的相关性。
Near-infrared Raman spectroscopy is a powerful analytical tool for detecting critical differences in biological samples with minimum interference in the Raman spectra from the native fluorescence of the samples. The technique is often suggested as a potential screening tool for cancer. In this article we report in vitro Raman spectra of squamous cells in normal and cancerous cervical human tissue from seven patients, which have good signal-to-noise ratio and which were found to be reproducible. These preliminary results show that several Raman features in these spectra could be used to distinguish cancerous cervical squamous cells from normal cervical squamous cells. In general, the Raman spectra of cervical cancer cells show intensity differences compared to those of normal squamous cell spectra. For example, several well-defined Raman peaks of collagen in the 775 to 975 cm −1 region are observed in the case of normal squamous cells, but these are below the detection limit of normal Raman spectroscopy in the spectra of invasive cervical cancer cells. In the high frequency 2800 to 3100 cm −1 region, it is found that the peak area under the CH stretching band is lower by a factor of approximately six in the spectra of cervical cancer cells as compared with that of the normal cells. The Raman chemical maps of regions of cancer and normal cells in the cervical epithelium made from the spectral features in the 775 to 975 cm −1 and 2800 to 3100 cm −1 regions are also found to show good correlation with each other.
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DOI：
10.1016/j.saa.2005.02.020
发表时间：
2005-08-01
影响因子：
4.4
作者：
Sharma, SK;Misra, AK;Sharma, B
通讯作者：
Sharma, B
影响因子：
254.7
作者：
Parkin, DM;Bray, F;Pisani, P
通讯作者：
Pisani, P
影响因子：
2.5
作者：
de Jong, BWD;Schut, TCB;Puppels, GJ
通讯作者：
Puppels, GJ
影响因子：
2.5
作者：
Krishna, CM;Prathima, NB;Kartha, VB
通讯作者：
Kartha, VB
影响因子：
3.6
作者：
Lyng, F. M.;Faolain, E. O.;Byrne, H. J.
通讯作者：
Byrne, H. J.