Axon regeneration through scaffold into distal spinal cord after transection.
Axon regeneration through scaffold into distal spinal cord after transection.
复制标题
横断后，通过支架再生到脊髓远端的轴突再生。
DOI：
10.1089/neu.2008-0610
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发表时间：
2009-10
影响因子：
4.2
通讯作者：
Windebank AJ
中科院分区：
文献类型：
--
作者：
Chen BK;Knight AM;de Ruiter GC;Spinner RJ;Yaszemski MJ;Currier BL;Windebank AJ
我们采用固蓝（FB）轴突追踪来确定大鼠胸脊髓横断损伤后再生轴突的起源。横断后植入负载雪旺细胞（SC）、可生物降解的聚（乳酸-乙醇酸）（PLGA）支架。负载有溶解的基底膜制剂（不含 SC）的支架用作阴性对照，未横断的绳索用作阳性对照。受伤和支架植入后 1 或 2 个月，将 FB 注射到支架尾侧 0-15 mm 或距支架约 5 mm 的位置。一周后，收获组织并在低温恒温器上纵向（30 μm）切开支架和绳索。在所有细胞移植组中，用共聚焦显微镜鉴定了神经元细胞体的跨支架标记。大（直径 30-50 μm）神经元细胞体主要标记在腹角区域。大多数标记的神经元位于支架吻端 1-10 毫米处，尽管一些神经元也在颈髓中被标记。脊髓横断后，轴突生长双向发生，轴突再生至 PLGA 支架外 14 毫米并进入远端脊髓。 FB 标记的程度与注射部位到支架的距离呈负相关。植入后2个月，电子显微镜显示植入支架的横截面中有髓鞘轴突。具有细胞外胶原和基底层的髓鞘形成模式是 SC 髓鞘形成的特征。我们的结果表明 FB 标记是测量再生轴突起源的有效方法。
We employed Fast Blue (FB) axonal tracing to determine the origin of regenerating axons after thoracic spinal cord transection injury in rats. Schwann cell (SC)-loaded, biodegradable, poly(lactic-co-glycolic acid) (PLGA) scaffolds were implanted after transection. Scaffolds loaded with solubilized basement membrane preparation (without SCs) were used for negative controls, and nontransected cords were positive controls. One or 2 months after injury and scaffold implantation, FB was injected 0–15 mm caudal or about 5 mm rostral to the scaffold. One week later, tissue was harvested and the scaffold and cord sectioned longitudinally (30 μm) on a cryostat. Trans-scaffold labeling of neuron cell bodies was identified with confocal microscopy in all cell-transplanted groups. Large (30–50 μm diameter) neuron cell bodies were predominantly labeled in the ventral horn region. Most labeled neurons were seen 1–10 mm rostral to the scaffold, although some neurons were also labeled in the cervical cord. Axonal growth occurred bidirectionally after cord transection, and axons regenerated up to 14 mm beyond the PLGA scaffolds and into distal cord. The extent of FB labeling was negatively correlated with distance from the injection site to the scaffold. Electron microscopy showed myelinated axons in the transverse sections of the implanted scaffold 2 months after implantation. The pattern of myelination, with extracellular collagen and basal lamina, was characteristic of SC myelination. Our results show that FB labeling is an effective way to measure the origin of regenerating axons.
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DOI：
10.1073/pnas.80.12.3850
发表时间：
1983-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CORNBROOKS, CJ;CAREY, DJ;BUNGE, RP
通讯作者：
BUNGE, RP
影响因子：
7.8
作者：
DANILOFF, JK;LEVI, G;EDELMAN, GM
通讯作者：
EDELMAN, GM
DOI：
10.1682/jrrd.2003.08.0055
发表时间：
2003-07-01
影响因子：
--
作者：
Bunge, MB;Pearse, DD
通讯作者：
Pearse, DD
影响因子：
4.4
作者：
BRESNAHAN, JC
通讯作者：
BRESNAHAN, JC
影响因子：
3.1
作者：
Bamber NI;Li H;Aebischer P;Xu XM
通讯作者：
Xu XM