Yinchen Linggui Zhugan Decoction Ameliorates Nonalcoholic Fatty Liver Disease in Rats by Regulating the Nrf2/ARE Signaling Pathway.
Yinchen Linggui Zhugan Decoction Ameliorates Nonalcoholic Fatty Liver Disease in Rats by Regulating the Nrf2/ARE Signaling Pathway.
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DOI：
10.1155/2017/6178358
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Han HX
中科院分区：
文献类型：
--
作者：
Guo Y;Li JX;Wang YL;Mao TY;Chen C;Xie TH;Han YF;Tan X;Han HX
银陈灵归专肝汤是由两种著名的中药处方灵归专肝（LGZGD）和银陈好煎剂（YCHD）结合而成。在前期研究中，我们发现银陈灵归助肝汤（YCLGZGD）可调节非酒精性脂肪性肝病（NAFLD）病理过程中的脂质代谢紊乱，减轻炎症反应。然而，确切的潜在机制尚不清楚。本研究旨在探讨银陈灵归助肝汤对高脂饮食大鼠实验性NAFLD的影响及其机制。每天给药YCLGZGD、LGZGD和YCHD，连续4周后安乐死。通过血脂、肝酶、H&E和油红O染色来评估NAFLD的严重程度。采用Western blotting和实时聚合酶链反应分别检测Keap1、Nrf2、NQO1、HO-1的肝脏蛋白及基因表达。口服YCLGZGD可改善hfd诱导的NAFLD。此外，YCLGZGD增加了Nrf2、NQO1和HO-1的蛋白和基因表达，但没有改变Keap1。总之，这些结果表明YCLGZGD通过上调Nrf2/ARE信号通路改善hfd诱导的大鼠NAFLD。
Yinchen Linggui Zhugan Decoction (YCLGZGD) is the combination of Linggui Zhugan (LGZGD) and Yinchenhao (YCHD) decoctions, two famous traditional Chinese medicine prescriptions. In previous studies, we found that Yinchen Linggui Zhugan Decoction (YCLGZGD) could regulate lipid metabolism disorder and attenuate inflammation in pathological process of nonalcoholic fatty liver disease (NAFLD). However, the exact underlying mechanism remains unknown. The aim of this study was to explore the effect of Yinchen Linggui Zhugan Decoction on experimental NAFLD and its mechanism in rats with high-fat diet (HFD) which was established by 8-week administration of HFD. YCLGZGD, LGZGD, and YCHD were administered daily for 4 weeks, after which the rats were euthanized. The level of blood lipid, liver enzymes, H&E, and Oil Red O staining were determined to evaluate NAFLD severity. Western blotting and real-time polymerase chain reaction were, respectively, used to determine hepatic protein and gene expression of Keap1, Nrf2, NQO1, and HO-1. Oral YCLGZGD ameliorated HFD-induced NAFLD. Furthermore, YCLGZGD increased the protein and gene expression of Nrf2, NQO1, and HO-1 without changing Keap1. Overall, these results suggest that YCLGZGD ameliorates HFD-induced NAFLD in rats by upregulating the Nrf2/ARE signaling pathway.
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影响因子：
10.5
作者：
Itoh, K;Wakabayashi, N;Yamamoto, M
通讯作者：
Yamamoto, M
非酒精性脂肪肝病的发病机理。
DOI：
10.1093/qjmed/hcp158
发表时间：
2010-02
期刊：
QJM : monthly journal of the Association of Physicians
影响因子：
--
作者：
Dowman JK;Tomlinson JW;Newsome PN
通讯作者：
Newsome PN
影响因子：
5.6
作者：
Pan, Chen-Wei;Zhou, Guang-yao;Pan, Zhen-zhen
通讯作者：
Pan, Zhen-zhen
影响因子：
3.3
作者：
Bahabadi, Majid;Mohammadalipour, Adel;Khodadadi, Iraj
通讯作者：
Khodadadi, Iraj
影响因子：
5.7
作者：
Bao, Jiaolin;Ding, Renbo;He, Chengwei
通讯作者：
He, Chengwei