Extracellular proteome analysis of Leptospira interrogans serovar Lai.
Extracellular proteome analysis of Leptospira interrogans serovar Lai.
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问号钩端螺旋体赖血清型的细胞外蛋白质组分析。
DOI：
10.1089/omi.2013.0043
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发表时间：
2013-09
期刊：
影响因子：
--
通讯作者：
Qin, Jinhong
中科院分区：
文献类型：
--
作者：
Zeng, Lingbing;Zhang, Yunyi;Zhu, Yongzhang;Yin, Haidi;Zhuang, Xuran;Zhu, Weinan;Guo, Xiaokui;Qin, Jinhong
钩端螺旋体病是最重要的人畜共患病之一。钩端螺旋体是引起钩端螺旋体病的致病性螺旋体。胞外蛋白在这种细菌的致病性中起重要作用。在本研究中，在无蛋白培养基中培养审问乳杆菌血清型Lai；收集上清，随后作为细胞外蛋白质组进行分析。MS/MS共检测到66个具有2个以上独特肽的蛋白，其中33个通过生物信息学分析预测为细胞外蛋白，包括Psortb、cello、SoSuiGramN和SignalP。比较这33个基因在体内和体外的转录水平，发现15个基因在体内与体外相比表达上调，2个基因表达下调。在基因组和蛋白质组学水平上对分泌系统成分进行BLAST搜索，发现该菌株存在完整的I型分泌系统和II型分泌系统。此外，该菌株还具有完整的Sec转位酶和Tat转位酶系统。对钩端螺旋体的细胞外蛋白质组学分析将为研究钩端螺旋体感染过程中特定蛋白质的功能以及选择疫苗或诊断工具的候选分子提供更多信息。
Abstract Leptospirosis is one of the most important zoonoses. Leptospira interrogans serovar Lai is a pathogenic spirochete that is responsible for leptospirosis. Extracellular proteins play an important role in the pathogenicity of this bacterium. In this study, L. interrogans serovar Lai was grown in protein-free medium; the supernatant was collected and subsequently analyzed as the extracellular proteome. A total of 66 proteins with more than two unique peptides were detected by MS/MS, and 33 of these were predicted to be extracellular proteins by a combination of bioinformatics analyses, including Psortb, cello, SoSuiGramN and SignalP. Comparisons of the transcriptional levels of these 33 genes between in vivo and in vitro conditions revealed that 15 genes were upregulated and two genes were downregulated in vivo compared to in vitro. A BLAST search for the components of secretion system at the genomic and proteomic levels revealed the presence of the complete type I secretion system and type II secretion system in this strain. Moreover, this strain also exhibits complete Sec translocase and Tat translocase systems. The extracellular proteome analysis of L. interrogans will supplement the previously generated whole proteome data and provide more information for studying the functions of specific proteins in the infection process and for selecting candidate molecules for vaccines or diagnostic tools for leptospirosis.
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影响因子：
4.2
作者：
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通讯作者：
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影响因子：
3.4
作者：
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影响因子：
3.7
作者：
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影响因子：
6.7
作者：
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通讯作者：
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影响因子：
44.1
作者：
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