Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers.
Organic zinc with moderate chelation strength enhances zinc absorption in the small intestine and expression of related transporters in the duodenum of broilers.
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DOI：
10.3389/fphys.2022.952941
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发表时间：
2022
影响因子：
4
通讯作者：
Luo, Xugang
中科院分区：
文献类型：
--
作者：
Hu, Yun;Wang, Chuanlong;Wu, Wei;Qu, Yicheng;Zhang, Weiyun;Li, Ding;Zhu, Ling;Gao, Feiyu;Wu, Bingxin;Zhang, Liyang;Cui, Xiaoyan;Li, Tingting;Geng, Yanqiang;Luo, Xugang
关键词：
本实验室前期的研究表明，在原位结扎的肉鸡十二指肠段中，中等螯合强度的有机锌蛋白对锌的吸收显著高于无机硫酸锌，但其机制尚不清楚。本研究旨在探讨中等螯合强度的有机锌和无机锌对肉鸡小肠锌吸收及十二指肠相关转运蛋白表达的影响。试验选用13日龄缺锌肉鸡，分别饲喂经分析含锌25.72和25.64 mg/kg的不加锌基础日粮（对照）或添加60 mg/kg锌的硫酸锌或中等螯合强度锌蛋白盐（Zn-Prot M），试验期26 d。结果表明，在28日龄和39日龄时，添加锌可显著提高肉鸡肝门静脉血锌含量（p < 0.05），且锌蛋白M组显著高于硫酸锌组（p < 0.05）。在第28天，锌添加上调（p < 0.05）锌转运蛋白1（ZnT 1），Zrt-irt-like蛋白5（ZIP 5），y + L-型氨基转运蛋白2（y + LAT 2）和b 0，+型氨基酸转运蛋白（rBAT）的mRNA表达，锌转运蛋白4（ZnT 4）的蛋白表达，锌转运蛋白9（ZnT 9）的mRNA和蛋白表达。此外，Zn-Prot M组肉鸡十二指肠中ZnT 9 mRNA表达、ZnT 4、ZIP 5和rBAT蛋白表达、锌转运蛋白7（ZnT 7）和y + LAT 2 mRNA和蛋白表达均高于硫酸锌组（p < 0.05）。在第39天，补锌组十二指肠中肽转运蛋白1（PepT 1）mRNA表达和y + LAT 2蛋白表达显著增加（p < 0.05），而Zn-Prot M组的ZnT 7和Zrt-irt-like protein 3（ZIP 3）mRNA表达显著高于硫酸锌组（p < 0.05）。因此，Zn-Prot M可促进肉鸡小肠锌的吸收，其作用机制可能是通过上调十二指肠ZnT 4、ZnT 7、ZnT 9、ZIP 3、ZIP 5、γ + LAT 2和rBAT的表达而实现的。
Our previous study demonstrated that the absorption of zinc (Zn) from the organic Zn proteinate with moderate chelation strength was significantly higher than that of Zn from the inorganic Zn sulfate in the in situ ligated duodenal segment of broilers, but the underlying mechanisms are unknown. The present study aimed to determine the effect of organic Zn with moderate chelation strength and inorganic Zn on the Zn absorption in the small intestine and the expression of related transporters in the duodenum of broilers. The Zn-deficient broilers (13 days old) were fed with the Zn-unsupplemented basal diets (control) containing 25.72 and 25.64 mg Zn/kg by analysis or the basal diets supplemented with 60 mg Zn/kg as the Zn sulfate or the Zn proteinate with moderate chelation strength (Zn-Prot M) for 26 days. The results showed that the plasma Zn contents from the hepatic portal vein of broilers at 28 days and 39 days of age were increased (p < 0.05) by Zn addition and greater (p < 0.05) in the Zn-Prot M than in the Zn sulfate. On d 28, Zn addition upregulated (p < 0.05) mRNA expression of zinc transporter 1 (ZnT1), Zrt-irt-like protein 5 (ZIP5), y + L-type amino transporter 2 (y + LAT2) and b0,+-type amino acid transporter (rBAT), zinc transporter 4 (ZnT4) protein expression, and zinc transporter 9 (ZnT9) mRNA and protein expression in the duodenum. Moreover, ZnT9 mRNA expression, ZnT4, ZIP5, and rBAT protein expression, zinc transporter 7 (ZnT7), and y + LAT2 mRNA and protein expression in the duodenum of broilers on 28 days were higher (p < 0.05) in the Zn-Prot M than in the Zn sulfate. On d 39, supplemental Zn increased (p < 0.05) peptide-transporter 1 (PepT1) mRNA expression and y + LAT2 protein expression, while the mRNA expression of ZnT7 and Zrt-irt-like protein 3 (ZIP3) were higher (p < 0.05) for the Zn-Prot M than for the Zn sulfate in the duodenum. It was concluded that the Zn-Prot M enhanced the Zn absorption in the small intestine partially via upregulating the expression of ZnT4, ZnT7, ZnT9, ZIP3, ZIP5, y + LAT2, and rBAT in the duodenum of broilers.
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饮食锌来源会影响年轻肉鸡的肠形态和氧化应激。
影响因子：
4.4
作者：
De Grande A;Leleu S;Delezie E;Rapp C;De Smet S;Goossens E;Haesebrouck F;Van Immerseel F;Ducatelle R
通讯作者：
Ducatelle R
影响因子：
4.4
作者：
Huang, Y. L.;Lu, L.;Liu, B.
通讯作者：
Liu, B.
影响因子：
3.3
作者：
Huang, Y. L.;Lu, L.;Liu, B.
通讯作者：
Liu, B.
影响因子：
3.7
作者：
Jando, Julia;Camargo, Simone M. R.;Verrey, Francois
通讯作者：
Verrey, Francois
影响因子：
6.1
作者：
Gao, Song;Yin, Taijun;Hu, Ming
通讯作者：
Hu, Ming