Biological effects of inhaled hydraulic fracturing sand dust. VIII. Immunotoxicity.
Biological effects of inhaled hydraulic fracturing sand dust. VIII. Immunotoxicity.
复制标题
DOI：
10.1016/j.taap.2020.115256
复制
发表时间：
2020-12-01
影响因子：
3.8
通讯作者：
Fedan JS
中科院分区：
文献类型：
--
作者：
Anderson SE;Shane H;Long C;Marrocco A;Lukomska E;Roberts JR;Marshall N;Fedan JS
水力压裂（“水力压裂”）是一种通过在压力下压裂地下天然气岩石来增强天然气回收的过程。用于稳定裂缝和促进气体流动的沙子会产生可吸入压裂沙尘 (FSD) 的潜在职业危害。由于缺乏对 FSD 免疫毒性的研究，因此在大鼠暴露后 1、7 和 27 天研究了 FSD（即 FSD 8）全身吸入（每天 6 小时，持续 4 天）的影响。暴露于 10 mg/m3 FSD 8 后 7 天，导致肺相关淋巴结 (LLN) 细胞结构、总 B 细胞、CD4+ T 细胞、CD8+ T 细胞和总自然杀伤 (NK) 细胞减少。在 LLN 中，CD4+ T 细胞的频率下降，而 B 细胞的频率增加（7 和 27 天）。相反，在暴露后 1 d 30 mg/m3 FSD 8 后，大鼠中观察到 LLN 细胞结构增加以及总 CD4+ 和 CD8+ T 细胞增加。在暴露后 7 天（10 mg/m3）时，观察到支气管肺泡灌洗液中 CD4+ T 细胞和 NK 细胞的频率和数量增加，以及暴露后 1 天（30 mg/m3）时总 CD4+ T 细胞、CD11b + 细胞和 NK 细胞的增加。暴露于 30 mg/m3 FSD 8 后 1 天，观察到脾脏中 B 细胞和 CD8+ T 细胞数量增加。此外，暴露后 1 天（30 mg/m3）和 27 天（10 mg/m3）时 NK 细胞活性受到抑制。未观察到对绵羊红细胞的 IgM 反应发生变化。研究结果表明，FSD 8 引起细胞结构、表型亚群的改变和免疫功能的损害。
Hydraulic fracturing (“fracking”) is a process used to enhance retrieval of gas from subterranean natural gasladen rock by fracturing it under pressure. Sand used to stabilize fissures and facilitate gas flow creates a potential occupational hazard from respirable fracking sand dust (FSD). As studies of the immunotoxicity of FSD are lacking, the effects of whole-body inhalation (6 h/d for 4 d) of a FSD, i.e., FSD 8, was investigated at 1, 7, and 27 d post-exposure in rats. Exposure to 10 mg/m3 FSD 8 resulted in decreased lung-associated lymph node (LLN) cellularity, total B-cells, CD4+ T-cells, CD8+ T-cells and total natural killer (NK) cells at 7-d post exposure. The frequency of CD4+ T-cells decreased while the frequency of B-cells increased (7 and 27 d) in the LLN. In contrast, increases in LLN cellularity and increases in total CD4+ and CD8+ T-cells were observed in rats following 30 mg/m3 FSD 8 at 1 d post-exposure. Increases in the frequency and number of CD4+ T-cells and NK cells were observed in bronchial alveolar lavage fluid at 7-d post-exposure (10 mg/m3) along with an increase in total CD4+ T-cells, CD11b + cells, and NK cells at 1-day post-exposure (30 mg/m3). Increases in the numbers of B-cells and CD8+ T-cells were observed in the spleen at 1-day post 30 mg/m3 FSD 8 exposure. In addition, NK cell activity was suppressed at 1 d (30 mg/m3) and 27 d post-exposure (10 mg/m3). No change in the IgM response to sheep red blood cells was observed. The findings indicate that FSD 8 caused alterations in cellularity, phenotypic subsets, and impairment of immune function.
登录
查看更多内容
影响因子：
7.3
作者：
Nishijima N;Hirai T;Misato K;Aoyama M;Kuroda E;Ishii KJ;Higashisaka K;Yoshioka Y;Tsutsumi Y
通讯作者：
Tsutsumi Y
影响因子：
5.5
作者：
Beamer, Celine A.;Migliaccio, Christopher T.;Holian, Andrij
通讯作者：
Holian, Andrij
DOI：
10.1080/15287394.2015.1056898
发表时间：
2015-09-02
影响因子：
2.6
作者：
Marrocco, Antonella;Meade, B. Jean;Anderson, Stacey E.
通讯作者：
Anderson, Stacey E.
影响因子：
10
作者：
Sayan M;Mossman BT
通讯作者：
Mossman BT
影响因子：
3.8
作者：
DeWitt, Jamie;Buck, Brenda;Keil, Deborah
通讯作者：
Keil, Deborah