The form of nitrogen nutrition affects resistance against Pseudomonas syringae pv. phaseolicola in tobacco.
The form of nitrogen nutrition affects resistance against Pseudomonas syringae pv. phaseolicola in tobacco.
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DOI：
10.1093/jxb/ers348
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发表时间：
2013-01
影响因子：
6.9
通讯作者：
Mur LA
中科院分区：
文献类型：
--
作者：
Gupta KJ;Brotman Y;Segu S;Zeier T;Zeier J;Persijn ST;Cristescu SM;Harren FJ;Bauwe H;Fernie AR;Kaiser WM;Mur LA
不同形式的氮（N）肥料影响疾病的发展;然而，本研究调查了N形式对超敏反应（HR）的影响-病原体引起的细胞死亡与抗性有关。产氢假单胞菌致病变种将菜豆内生真菌渗入到用或饲喂的烟草叶片中。细胞死亡的速度快，在饥饿的植物相比，相关，分别与增加和减少的阻力。硝酸还原酶（NR）可以产生一氧化氮（NO），影响HR的形成。在氮同化绕过NR步骤的植物中，NO的产生减少。这与致病性P. degeniae pv.烟粉虱菌株，进一步表明抗性与喂养妥协。PR 1a是防御信号水杨酸（SA）的生物标志物，并且在接种后24小时，与饲喂植物相比，在饲喂中表达降低。该模式与实际SA测量值相关。相反，总氨基酸，胞质和质外体葡萄糖/果糖和蔗糖升高处理植物。气相色谱/质谱法被用来表征不同的N处理后的代谢事件。营养后，多胺的生物合成是占主导地位的，而营养后，通量似乎转向生产4-氨基丁酸。喂养增强SA，NO，和多胺介导的HR相关的防御，而这些都受到损害，这也增加了营养物质的可用性，病原体的机制进行了讨论。
Different forms of nitrogen (N) fertilizer affect disease development; however, this study investigated the effects of N forms on the hypersensitivity response (HR)—a pathogen-elicited cell death linked to resistance. HR-eliciting Pseudomonas syringae pv. phaseolicola was infiltrated into leaves of tobacco fed with either or . The speed of cell death was faster in -fed compared with -fed plants, which correlated, respectively, with increased and decreased resistance. Nitric oxide (NO) can be generated by nitrate reductase (NR) to influence the formation of the HR. NO generation was reduced in -fed plants where N assimilation bypassed the NR step. This was similar to that elicited by the disease-forming P. syringae pv. tabaci strain, further suggesting that resistance was compromised with feeding. PR1a is a biomarker for the defence signal salicylic acid (SA), and expression was reduced in -fed compared with fed plants at 24h after inoculation. This pattern correlated with actual SA measurements. Conversely, total amino acid, cytosolic and apoplastic glucose/fructose and sucrose were elevated in - treated plants. Gas chromatography/mass spectroscopy was used to characterize metabolic events following different N treatments. Following nutrition, polyamine biosynthesis was predominant, whilst after nutrition, flux appeared to be shifted towards the production of 4-aminobutyric acid. The mechanisms whereby feeding enhances SA, NO, and polyamine-mediated HR-linked defence whilst these are compromised with , which also increases the availability of nutrients to pathogens, are discussed.
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影响因子：
64.8
作者：
Delledonne, M;Xia, YJ;Lamb, C
通讯作者：
Lamb, C
影响因子：
2.1
作者：
Cristescu, S. M.;Persijn, S. T.;Harren, F. J. M.
通讯作者：
Harren, F. J. M.
影响因子：
7.4
作者：
Elisa Gonzalez, Maria;Marco, Francisco;Luis Pieckenstain, Fernando
通讯作者：
Luis Pieckenstain, Fernando
影响因子：
3
作者：
Arvidsson S;Kwasniewski M;Riaño-Pachón DM;Mueller-Roeber B
通讯作者：
Mueller-Roeber B
影响因子：
7.3
作者：
Fritz, Christina;Mueller, Cathrin;Stitt, Mark
通讯作者：
Stitt, Mark