Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis
Isolation and characterization of a MADS-box gene in cucumber (Cucumis sativus L.) that affects flowering time and leaf morphology in transgenic Arabidopsis
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黄瓜 (Cucumis sativus L.) 中影响转基因拟南芥开花时间和叶片形态的 MADS-box 基因的分离和表征
DOI：
10.1080/13102818.2018.1534556
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发表时间：
2019-01
影响因子：
1.4
通讯作者：
Liu Shiqiang
中科院分区：
文献类型：
--
作者：
Zhou Yong;Hu Lifang;Song Jianbo;Jiang Lunwei;Liu Shiqiang
MADS-box家族基因是在植物生长发育过程中起重要作用的重要转录因子。本研究在黄瓜（Cucumis sativus L.）中鉴定了一个MADS-box基因CsMADS02，并对其进行了功能鉴定。CsMADS02编码一个典型的SEP MADS-box蛋白，该蛋白包含一个MADS-box、一个K-box、一个I区和一个C区，以及两个短的保守SEP基序。序列比对和系统发育分析表明，CsMADS02蛋白属于SEP3亚家族，与其他已发表的植物SEP蛋白高度同源。表达分析表明，CsMADS02主要在花和子房中表达，在雄花和雌花发育过程中差异表达。CsMADS02异位过表达导致拟南芥提早开花。此外，转基因植株的形态也发生了变化，包括卷曲和较少的莲座叶。这些结果表明，CsMADS02是黄瓜开花时间和叶片形态的潜在调节剂。
Abstract The MADS-box family genes are important transcription factors that play essential roles in plant growth and development. In this study, a MADS-box gene, CsMADS02, was identified and functionally characterized in cucumber (Cucumis sativus L.). CsMADS02 encoded a typical SEP MADS-box protein which contained a MADS-box, a K-box, an I region and a C region, as well as two short conserved SEP motifs. Sequence alignment and phylogenetic analysis showed that the CsMADS02 protein belonged to the SEP3 subfamily and was highly homologous to other published plant SEP proteins. Expression analyses revealed that CsMADS02 was primarily expressed in flowers and ovaries, and differentially expressed during the development of male and female flowers. Ectopic overexpression of CsMADS02 caused earlier flowering of Arabidopsis. In addition, morphological changes were also observed in transgenic plants, including curled and fewer rosette leaves. These findings suggest that CsMADS02 is a potential regulator of the flowering time and leaf morphology in cucumber.
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