Proteomic analysis of porcine mesenchymal stem cells derived from bone marrow and umbilical cord: implication of the proteins involved in the higher migration capability of bone marrow mesenchymal stem cells.
Proteomic analysis of porcine mesenchymal stem cells derived from bone marrow and umbilical cord: implication of the proteins involved in the higher migration capability of bone marrow mesenchymal stem cells.
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源自骨髓和脐带的猪间充质干细胞的蛋白质组学分析：涉及骨髓间充质干细胞较高迁移能力的蛋白质的含义。
DOI：
10.1186/s13287-015-0061-x
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发表时间：
2015-04-15
影响因子：
7.5
通讯作者：
Li K
中科院分区：
文献类型：
--
作者：
Huang L;Niu C;Willard B;Zhao W;Liu L;He W;Wu T;Yang S;Feng S;Mu Y;Zheng L;Li K
间充质干细胞（Mesenchymal stem cells, MSCs）具有体内增殖能力和多种分化潜能，因此被广泛用作细胞治疗的理想材料。猪源间充质干细胞和人源间充质干细胞在许多方面相似，如细胞免疫表型和功能特征。然而，猪骨髓间充质干细胞（BM-MSCs）和脐带间充质干细胞（UC-MSCs）之间的蛋白质组学差异和细胞功能的分子机制在很大程度上是未知的。据我们所知，从不同组织中收集的间充质干细胞具有特定的表型和分化能力，以响应微环境，即生态位。用流式细胞术和成脂成骨分化分析对猪BM-MSCs和UC-MSCs进行评价。我们使用等压标记相对和绝对定量（iTRAQ），结合液相色谱-串联质谱法，鉴定这两种MSCs之间的差异表达蛋白（DEPs）。利用京都基因和基因组百科全书途径和表型分析来了解细胞迁移能力和dep之间的联系。进行了两次单独的iTRAQ实验，确定了95个dep（95%置信区间）。其中5种蛋白经Western blotting验证。从生物调控、代谢过程、发育过程、免疫系统过程、繁殖、死亡、生长、信号、定位、刺激反应、生物粘附和细胞组分组织等方面对这95种DEPs进行了分类。我们的研究首次表明，猪BM-MSCs比UC-MSCs具有更高的迁移能力。最后，其中一种DEPs， Vimentin，被证实在MSC迁移中具有积极作用。这些结果代表了首次尝试使用蛋白质组学专门针对不同组织的猪间充质干细胞。鉴定出的成分将有助于揭示组织来源的MSC群体中的各种组织特异性功能，并可作为未来使用动物模型的干细胞组织工程研究中特定组织再生的重要工具。本文的在线版本（doi:10.1186/s13287-015-0061-x）包含补充材料，仅供授权用户使用。
Mesenchymal stem cells (MSCs) have the ability to proliferate in vivo with a large variety of differentiation potentials and therefore are widely used as an ideal material for cell therapy. MSCs derived from pig and human sources are similar in many aspects, such as cell immunophenotype and functional characteristics. However, differences in proteomics and the molecular mechanisms of cell functions between porcine bone marrow MSCs (BM-MSCs) and umbilical cord MSCs (UC-MSCs) are largely unknown. To the best of our knowledge, MSCs collected from different tissue have specific phenotype and differentiation ability in response to microenvironment, known as a niche. Porcine BM-MSCs and UC-MSCs were evaluated with flow cytometric and adipogenic and osteogenic differentiation analyses. We used isobaric tagging for relative and absolute quantitation (iTRAQ), combined with liquid chromatography-tandem mass spectrometry, to identify differentially expressed proteins (DEPs) between these two types of MSCs. Kyoto Encyclopedia of Genes and Genomes pathway and phenotype analyses were used to understand the links between cell migration ability and DEPs. Two separate iTRAQ experiments were conducted, identifying 95 DEPs (95% confidence interval). Five of these proteins were verified by Western blotting. These 95 DEPs were classified in terms of biological regulation, metabolic process, developmental process, immune system process, reproduction, death, growth, signaling, localization, response to stimulus, biological adhesion, and cellular component organization. Our study is the first to show results indicating that porcine BM-MSCs have a higher migration capability than UC-MSCs. Finally, one of the DEPs, Vimentin, was verified to have a positive role in MSC migration. These results represent the first attempt to use proteomics specifically targeted to porcine MSCs of different tissues. The identified components should help reveal a variety of tissue-specific functions in tissue-derived MSC populations and could serve as important tools for the regeneration of particular tissues in future stem cell-based tissue engineering studies using animal models. The online version of this article (doi:10.1186/s13287-015-0061-x) contains supplementary material, which is available to authorized users.
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影响因子：
3.7
作者：
Burch TC;Watson MT;Nyalwidhe JO
通讯作者：
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32.4
作者：
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作者：
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通过 iTRAQ 标记结合在线二维 LC/MS/MS 对 C3H10T1/2 间充质干细胞的软骨分化进行定量蛋白质组学分析
影响因子：
7
作者：
Ji, Yu-hua;Ji, Ju-ling;Wu, Wei
通讯作者：
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