Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core.
Biochemical and biophysical characterization of the transmissible gastroenteritis coronavirus fusion core.
复制标题
可传染性胃炎冠状病毒融合核心的生化和生物物理表征。
DOI：
10.1016/j.bbrc.2005.09.189
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发表时间：
2005-12-02
影响因子：
3.1
通讯作者：
Li Y
中科院分区：
文献类型：
--
作者：
Ma G;Feng Y;Gao F;Wang J;Liu C;Li Y
传染性胃肠炎冠状病毒（TGEV）是引起哺乳仔猪肠道感染的主要病原之一，每年给养猪业造成巨大的经济损失。尽管多年来人们已经知道TGEV spiker蛋白是病毒进入所必需的，但对TGEV融合蛋白核心的详细了解仍然非常有限。本文报道了在GST原核表达系统中体外表达的TGEV融合核心（HR 1-SGGRGG-HR 2）具有典型的卷曲螺旋异二聚体三聚体（六个α-螺旋束）的性质，这一点已被一系列生物化学和生物物理学证据所证实，包括凝胶过滤、化学交叉和圆图。3D同源结构模型呈现了其最可能的结构，与记录的冠状病毒极其相似。TGEV spiker蛋白属于I类融合蛋白，其特征在于存在两个七重重复序列（HR）区域HR 1和HR 2，目前对截短的TGEV融合蛋白核心的了解有助于设计靶向TGEV与其宿主之间膜融合的小分子或多肽药物。
Transmissible gastroenteritis coronavirus (TGEV) is one of the most destructive agents, responsible for the enteric infections that are lethal for suckling piglets, causing enormous economic loss to the porcine fostering industry every year. Although it has been known that TGEV spiker protein is essential for the viral entry for many years, the detail knowledge of the TGEV fusion protein core is still very limited. Here, we report that TGEV fusion core (HR1-SGGRGG-HR2), in vitro expressed in GST prokaryotic expression system, shares the typical properties of the trimer of coiled-coil heterodimer (six α-helix bundle), which has been confirmed by a combined series of biochemical and biophysical evidences including size exclusion chromatography (gel-filtration), chemical crossing, and circular diagram. The 3D homologous structure model presents its most likely structure, extremely similar to those of the coronaviruses documented. Taken together, TGEV spiker protein belongs to the class I fusion protein, characterized by the existence of two heptad-repeat (HR) regions, HR1 and HR2, and the present knowledge about the truncated TGEV fusion protein core may facilitate in the design of the small molecule or polypeptide drugs targeting the membrane fusion between TGEV and its host.
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影响因子：
16
作者：
Weissenhorn, W;Carfi, A;Wiley, DC
通讯作者：
Wiley, DC
影响因子：
3.8
作者：
Wild, TF;Buckland, R
通讯作者：
Buckland, R
影响因子：
3.8
作者：
Yu, M;Wang, EX;Gao, GF
通讯作者：
Gao, GF
影响因子：
5.4
作者：
Schwegmann-Wessels, C;Zimmer, G;Herrler, G
通讯作者：
Herrler, G
来自可传染性胃炎病毒的中国分离物Th-98的ORF7区域的分子克隆和系统发育分析。
影响因子：
1.6
作者：
Yin JC;Ren XF;Li YJ
通讯作者：
Li YJ