Long-term subcutaneous microdialysis sampling and qRT-PCR of MCP-1, IL-6 and IL-10 in freely-moving rats.
Long-term subcutaneous microdialysis sampling and qRT-PCR of MCP-1, IL-6 and IL-10 in freely-moving rats.
复制标题
自由移动的大鼠中MCP-1，IL-6和IL-10的长期皮下微透析采样和QRT-PCR。
DOI：
10.1039/c0mb00059k
复制
发表时间：
2011-01
影响因子：
--
通讯作者：
Stenken JA
中科院分区：
文献类型：
--
作者：
von Grote EC;Venkatakrishnan V;Duo J;Stenken JA
Cytokines are important mediators of the wound healing response. However, sampling of cytokines from the interstitial fluid at a healing wound site in experimental animals is a challenge. Microdialysis sampling is an in vivo collection technique option for this purpose as it permits continuous sampling, while remaining contiguous with the wound microenvironment. The polymeric membrane of the microdialysis probe is a foreign material thus allowing a unique approach to sample cytokines generated during a foreign body response (FBR). The focus of these studies was to use microdialysis sampling to collect cytokines from a microdialysis probe implant site in a rat model of a FBR up to 6 days post implantation. Fluorescent bead-based immunoassays (Luminex™) were used to quantify monocyte chemoattractant protein-1 (MCP-1/CCL2), interleukin-6 (IL-6) and interleukin-10 (IL-10) in the dialysates. Quantitative reverse transcription - polymerase chain reaction (qRT-PCR) relative expression of the protein transcription was used as a means to cross-validate the protein measurements obtained via micorodialysis sampling. A histological examination of tissue was also preformed to assess the progression in leukocyte extravasation and collagen deposition surrounding implanted probes. Our findings demonstrate that in vivo microdialysis sampling can be used to collect temporal concentrations of cytokines which are consistent with wound healing and the development of a FBR.
登录
查看更多内容
影响因子：
2.9
作者：
DiPietro, LA;Reintjes, MG;Gamelli, RL
通讯作者：
Gamelli, RL
DOI：
10.1002/jbm.10043
发表时间：
2002-06-05
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Collier, TO;Anderson, JM
通讯作者：
Anderson, JM
DOI：
10.1163/156856206777346340
发表时间：
2006-01-01
影响因子：
3.6
作者：
Gretzer, Christina;Emanuelsson, Lena;Thomsen, Peter
通讯作者：
Thomsen, Peter
影响因子：
2.2
作者：
Favre, N;Bordmann, G;Rudin, W
通讯作者：
Rudin, W
影响因子：
4.9
作者：
Brodbeck, WG;Voskerician, G;Anderson, JM
通讯作者：
Anderson, JM