Niche-adaptation in plant-associated Bacteroidetes favours specialisation in organic phosphorus mineralisation.
Niche-adaptation in plant-associated Bacteroidetes favours specialisation in organic phosphorus mineralisation.
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与植物相关的拟杆菌门的生态位适应有利于有机磷矿化的专业化。
DOI：
10.1038/s41396-020-00829-2
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发表时间：
2021-04
期刊：
影响因子：
--
通讯作者：
Scanlan DJ
中科院分区：
文献类型：
--
作者：
Lidbury IDEA;Borsetto C;Murphy ARJ;Bottrill A;Jones AME;Bending GD;Hammond JP;Chen Y;Wellington EMH;Scanlan DJ
拟杆菌是植物微生物组中丰富的病原体抑制成员，其对根际磷动员有显着贡献，这是该生态位中常见的生长限制营养素。然而，支撑他们在这个利基成功的遗传特征在很大程度上仍然未知，特别是关于他们的磷获取策略。通过结合培养、多层组学和生化分析，我们首次发现所有植物相关拟杆菌都表达组成型磷酸酶活性，这与普遍拥有的独特磷酸酶PafA有关。这是第一次，我们还揭示了拟杆菌外膜SusCD样复合物的一个子集，通常与碳收购，和几个TonB依赖性转运蛋白，诱导Pi耗尽。此外，在响应磷酸盐消耗，在这项研究中使用的植物相关黄杆菌表达了许多以前的特点和新的蛋白质靶向有机磷。总的来说，这些酶表现出上级磷酸酶活性相比，植物相关的假单胞菌。重要的是，几种新的低Pi诱导型磷酸酶和转运蛋白属于拟杆菌属辅助基因组，并且是植物相关菌株的适应性基因组特征。总之，对植物微生物组的生态位适应似乎导致拟杆菌获得独特的磷清除位点，增强了它们的磷获取能力。这些特性可能使它们在根际取得成功，也为发展可持续农业提供了令人兴奋的途径。
Bacteroidetes are abundant pathogen-suppressing members of the plant microbiome that contribute prominently to rhizosphere phosphorus mobilisation, a frequent growth-limiting nutrient in this niche. However, the genetic traits underpinning their success in this niche remain largely unknown, particularly regarding their phosphorus acquisition strategies. By combining cultivation, multi-layered omics and biochemical analyses we first discovered that all plant-associated Bacteroidetes express constitutive phosphatase activity, linked to the ubiquitous possession of a unique phosphatase, PafA. For the first time, we also reveal a subset of Bacteroidetes outer membrane SusCD-like complexes, typically associated with carbon acquisition, and several TonB-dependent transporters, are induced during Pi-depletion. Furthermore, in response to phosphate depletion, the plant-associated Flavobacterium used in this study expressed many previously characterised and novel proteins targeting organic phosphorus. Collectively, these enzymes exhibited superior phosphatase activity compared to plant-associated Pseudomonas spp. Importantly, several of the novel low-Pi-inducible phosphatases and transporters, belong to the Bacteroidetes auxiliary genome and are an adaptive genomic signature of plant-associated strains. In conclusion, niche adaptation to the plant microbiome thus appears to have resulted in the acquisition of unique phosphorus scavenging loci in Bacteroidetes, enhancing their phosphorus acquisition capabilities. These traits may enable their success in the rhizosphere and also present exciting avenues to develop sustainable agriculture.
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DOI：
10.5455/aim.2012.20.167-173
发表时间：
2012-09
期刊：
Acta informatica medica : AIM : journal of the Society for Medical Informatics of Bosnia & Herzegovina : casopis Drustva za medicinsku informatiku BiH
影响因子：
--
作者：
U Gandhi N;B Chandra S
通讯作者：
B Chandra S
影响因子：
3.7
作者：
Le Blastier S;Hamels A;Cabeen M;Schille L;Tilquin F;Dieu M;Raes M;Matroule JY
通讯作者：
Matroule JY
影响因子：
64.8
作者：
Glenwright AJ;Pothula KR;Bhamidimarri SP;Chorev DS;Baslé A;Firbank SJ;Zheng H;Robinson CV;Winterhalter M;Kleinekathöfer U;Bolam DN;van den Berg B
通讯作者：
van den Berg B
影响因子：
5.1
作者：
Bauer, Margarete;Kube, Michael;Gloeckner, Frank Oliver
通讯作者：
Gloeckner, Frank Oliver
影响因子：
6.7
作者：
Cordell, Dana;White, Stuart
通讯作者：
White, Stuart