Genome-Wide Analysis of the GRF Family Reveals Their Involvement in Abiotic Stress Response in Cassava.
Genome-Wide Analysis of the GRF Family Reveals Their Involvement in Abiotic Stress Response in Cassava.
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作者：
Shang S;Wu C;Huang C;Tie W;Yan Y;Ding Z;Xia Z;Wang W;Peng M;Tian L;Hu W
一般调节因子（GRF）蛋白在植物生长、发育和对非生物胁迫的反应中起着重要的调节作用。然而，很少的信息是已知的木薯（木薯）的这个基因家族。本研究从木薯基因组中鉴定了15个MeGRF，并根据系统发育、保守基序和基因结构分析将其分为ε和非ε两大类。转录组学分析表明，11个MeGRFs在两个木薯基因型的茎、叶和贮藏根中具有组成性高表达。表达分析表明，大多数GRFs在低温、渗透、盐、脱落酸（阿坝）和H2O2处理下表现出转录变化。6个MeGRFs被发现普遍上调非生物胁迫，阿坝和H2O2处理，这可能是多个信号通路的汇合点。相互作用网络分析确定了18个可能的相互作用的MeGRF。本研究阐明了MeGRFs在木薯组织发育和非生物胁迫响应中的转录调控及相关信号转导机制。一些组成型表达，组织特异性，非生物胁迫响应的候选基因被确定为作物的进一步遗传改良。
GENERAL REGULATORY FACTOR (GRF) proteins play vital roles in the regulation of plant growth, development, and response to abiotic stress. However, little information is known for this gene family in cassava (Manihot esculenta). In this study, 15 MeGRFs were identified from the cassava genome and were clustered into the ε and the non-ε groups according to phylogenetic, conserved motif, and gene structure analyses. Transcriptomic analyses showed eleven MeGRFs with constitutively high expression in stems, leaves, and storage roots of two cassava genotypes. Expression analyses revealed that the majority of GRFs showed transcriptional changes under cold, osmotic, salt, abscisic acid (ABA), and H2O2 treatments. Six MeGRFs were found to be commonly upregulated by abiotic stress, ABA, and H2O2 treatments, which may be the converging points of multiple signaling pathways. Interaction network analysis identified 18 possible interactors of MeGRFs. Taken together, this study elucidates the transcriptional control of MeGRFs in tissue development and the responses of abiotic stress and related signaling in cassava. Some constitutively expressed, tissue-specific, and abiotic stress-responsive candidate MeGRF genes were identified for the further genetic improvement of crops.
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影响因子：
11.6
作者：
DEVETTEN, NC;LU, GH;FERL, RJ
通讯作者：
FERL, RJ
DOI：
10.1093/bioinformatics/btt248
发表时间：
2013-07-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Brown P;Baxter L;Hickman R;Beynon J;Moore JD;Ott S
通讯作者：
Ott S
影响因子：
4.3
作者：
Li, Xuyan;Dhaubhadel, Sangeeta
通讯作者：
Dhaubhadel, Sangeeta
影响因子：
5.6
作者：
Chandna, Ruby;Augustine, Rehna;Bisht, Naveen C.
通讯作者：
Bisht, Naveen C.
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.