Label-free discrimination of tumorigenesis stages using in vitro prostate cancer bone metastasis model by Raman imaging.
Label-free discrimination of tumorigenesis stages using in vitro prostate cancer bone metastasis model by Raman imaging.
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DOI：
10.1038/s41598-022-11800-w
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发表时间：
2022-05-16
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
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作者：
转移性前列腺癌在骨骼中定居，为骨转移铺平了道路，导致与预后差和发病率相关的骨骼并发症。本研究利用纳米粘土为基础的三维（3D）骨模拟体外模型，模拟前列腺癌骨转移，证明了拉曼成像在肿瘤发生的不同阶段区分癌细胞的可行性。利用主成分分析（PCA）对二维（2D）模型和三维骨模拟环境中的前列腺癌细胞进行了一项综合研究，比较了不同时间间隔内接受前列腺癌细胞的分类。我们的研究结果显示，前列腺癌细胞和3D骨模拟支架中培养的细胞在拉曼成像上存在明显的光谱差异，特别是在1002、1261、1444和1654 cm−1处，主要含有蛋白质和脂质信号。拉曼图捕捉肿瘤细胞转移过程中的亚细胞反应。通过聚类分析的拉曼特征提取允许识别图像中的特定细胞成分。这项工作首次证明了拉曼成像、PCA和聚类分析在区分转移性肿瘤发生不同阶段的癌细胞方面具有很大的潜力。
Metastatic prostate cancer colonizes the bone to pave the way for bone metastasis, leading to skeletal complications associated with poor prognosis and morbidity. This study demonstrates the feasibility of Raman imaging to differentiate between cancer cells at different stages of tumorigenesis using a nanoclay-based three-dimensional (3D) bone mimetic in vitro model that mimics prostate cancer bone metastasis. A comprehensive study comparing the classification of as received prostate cancer cells in a two-dimensional (2D) model and cancer cells in a 3D bone mimetic environment was performed over various time intervals using principal component analysis (PCA). Our results showed distinctive spectral differences in Raman imaging between prostate cancer cells and the cells cultured in 3D bone mimetic scaffolds, particularly at 1002, 1261, 1444, and 1654 cm−1, which primarily contain proteins and lipids signals. Raman maps capture sub-cellular responses with the progression of tumor cells into metastasis. Raman feature extraction via cluster analysis allows for the identification of specific cellular constituents in the images. For the first time, this work demonstrates a promising potential of Raman imaging, PCA, and cluster analysis to discriminate between cancer cells at different stages of metastatic tumorigenesis.
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影响因子：
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