Investigation into the controversial association of Streptococcus gallolyticus with colorectal cancer and adenoma.
Investigation into the controversial association of Streptococcus gallolyticus with colorectal cancer and adenoma.
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DOI：
10.1186/1471-2407-9-403
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发表时间：
2009-11-19
期刊：
影响因子：
3.8
通讯作者：
Abubaker F
中科院分区：
文献类型：
--
作者：
Abdulamir AS;Hafidh RR;Mahdi LK;Al-jeboori T;Abubaker F
评价了溶没食子链球菌亚种（CIP 105428）IgG抗体的血清阳性率，以调查存在争议的溶没食子链球菌与链球菌感染的关系。通过检测NF-κB和IL-8的mRNA表达，试图研究这种关联的性质（如果有的话）。此外，S.将溶没食子酸杆菌IgG抗体与肠指示细菌脆弱拟杆菌的IgG抗体进行比较。用ELISA法检测血清中的IgG抗体。解没食子酸和B.在50名结直肠癌患者、14名结直肠腺瘤患者、30名年龄和性别匹配的表面健康志愿者（HV）和30名年龄和性别匹配的结肠镜检查证实的无肿瘤对照受试者的血清中测定了脆性链球菌。应用原位杂交技术检测癌和腺瘤患者肿瘤和非肿瘤组织中NF-κB和IL-8 mRNA的表达，并与对照组进行比较。结直肠癌和腺瘤患者血清S水平较高。溶血性胆固醇IgG抗体与HV和对照组比较有显著性差异（P < 0.05），而与血清B IgG抗体无相关性。脆性（P > 0.05）。ELISA检测S.从无肿瘤对照组计算解没食子酸IgG。NF-κB mRNA在腺瘤和癌组织中的表达高于非肿瘤组织，在癌/腺瘤组织中的表达高于对照组，在癌组织中的表达高于腺瘤患者，在S.在肿瘤组和非肿瘤组中，血清IgG阳性组均显著高于血清阴性组（P < 0.05）。IL-8 mRNA在腺瘤和癌的肿瘤切片中的表达高于非肿瘤切片，在癌/腺瘤中高于对照组，在S.在肿瘤组和非肿瘤组中，血清IgG阳性组显著高于血清阴性组（P < 0.05）。S.解没食子酸杆菌很可能在正常结肠直肠粘膜向腺瘤和CRC的致癌进展中起重要作用。S.解没食子酸可能通过利用某些炎性、抗凋亡和血管生成转化因子（包括NF-κB和IL-8）而发生。
The seroprevalence of IgG antibodies of Streptococcus gallolyticus subspecies gallolyticus, CIP 105428, was evaluated to investigate the controversial association of S. gallolyticus with colorectal carcinoma and adenoma in attempt to investigate the nature of such association if any, by exploring the mRNA expression of NF-κB and IL-8. Moreover, the serological behavior of S. gallolyticus IgG antibodies was compared to that of an indicator bacterium of bowel, Bacteroides fragilis. ELISA was used to measure IgG antibodies of S. gallolyticus and B. fragilis in sera of 50 colorectal cancer, 14 colorectal adenoma patients, 30 age- and sex- matched apparently healthy volunteers (HV) and 30 age- and sex- matched colonoscopically-proven tumor-free control subjects. NF-κB and IL-8 mRNA expression was evaluated in tumorous and non-tumorous tissue sections of carcinoma and adenoma patients in comparison with that of control subjects by using in situ hybridization assay. Colorectal cancer and adenoma patients were associated with higher levels of serum S. gallolyticus IgG antibodies in comparison with HV and control subjects (P < 0.05) while no similar association was found with serum IgG antibodies of B. fragilis (P > 0.05). ELISA cutoff value for the seropositivity of S. gallolyticus IgG was calculated from tumor-free control group. The expression of NF-κB mRNA was higher in tumorous than non-tumorous tissue sections of adenoma and carcinoma, higher in carcinoma/adenoma sections than in control subjects, higher in tumorous sections of carcinoma than in adenoma patients, and higher in S. gallolyticus IgG seropositive than in seronegative groups in both tumorous and non-tumorous sections (P < 0.05). IL-8 mRNA expression in tumorous sections of adenoma and carcinoma was higher than in non-tumorous sections, higher in carcinoma/adenoma than in control subjects, and higher in S. gallolyticus IgG seropositive than in seronegative groups in tumorous rather than non-tumorous sections (P < 0.05). S. gallolyticus most likely plays an essential role in the oncogenic progression of normal colorectal mucosa to adenoma and to CRC. This promoting/propagating role of S. gallolyticus might take place by utilizing certain inflammatory, anti-apoptotic, and angiogenic factors of transformation including NF-κB and IL-8.
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影响因子：
3.4
作者：
DARJEE, R;GIBB, AP
通讯作者：
GIBB, AP
影响因子：
4.7
作者：
Ellmerich, S;Schöller, M;Raul, F
通讯作者：
Raul, F
影响因子：
3.1
作者：
DELAHOOKE, DM;BARCLAY, GR;POXTON, IR
通讯作者：
POXTON, IR
DOI：
10.1016/0027-5107(94)90245-3
发表时间：
1994-03-01
期刊：
MUTATION RESEARCH
影响因子：
--
作者：
OHSHIMA, H;BARTSCH, H
通讯作者：
BARTSCH, H
影响因子：
6.4
作者：
Newman, JV;Kosaka, T;Schauer, DB
通讯作者：
Schauer, DB