Correlation of transient adenosine release and oxygen changes in the caudate-putamen.
Correlation of transient adenosine release and oxygen changes in the caudate-putamen.
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DOI：
10.1111/jnc.13705
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发表时间：
2017-01
影响因子：
4.7
通讯作者：
Venton BJ
中科院分区：
文献类型：
--
作者：
Wang Y;Venton BJ
腺苷是一种内源性核苷，可调节中枢神经系统中的重要生理过程，如血管舒张。最近发现了一种快速的2-4秒腺苷信号传导模式，但这种类型的腺苷与血流变化之间的关系尚未得到表征。在这项研究中，使用快速扫描循环伏安法同时测量了腺苷和氧的变化。当局部脑血流量增加时会发生氧变化，因此是血管舒张的一种量度。大鼠尾壳核中约34%的腺苷瞬变与随后的氧瞬变相关。氧的量与腺苷释放的浓度相关，并且较大的腺苷瞬变（超过0.4 μM）总是有随后的氧变化。腺苷和氧瞬变的平均持续时间分别为3.2秒和3.5秒。平均而言，腺苷释放开始并在氧气之前0.2秒达到峰值。A2 a拮抗剂SCH 442416使腺苷和氧瞬变事件的数量减少约32%。然而，A1拮抗剂，DPCPX，并没有显着影响同时腺苷和氧的释放。一氧化氮（NO）合酶抑制剂L-NAME也不影响腺苷和氧释放事件的浓度或数量。这些结果表明，腺苷和氧释放都是通过A2 a受体调节的。瞬时浓度、腺苷和氧峰之间的时间延迟以及A2 a受体的作用的相关性表明腺苷在快速的亚秒时间尺度上调节血流。
Adenosine is an endogenous nucleoside that modulates important physiological processes, such as vasodilation, in the central nervous system. A rapid, 2–4 seconds, mode of adenosine signaling has been recently discovered, but the relationship between this type of adenosine and blood flow change has not been characterized. In this study, adenosine and oxygen changes were simultaneously measured using fast-scan cyclic voltammetry. Oxygen changes occur when there is an increase in local cerebral blood flow and thus are a measure of vasodilation. About 34% of adenosine transients in the rat caudate-putamen are correlated with a subsequent transient change in oxygen. The amount of oxygen was correlated with the concentration of adenosine release and larger adenosine transients (over 0.4 μM) always had subsequent oxygen changes. The average duration of adenosine and oxygen transients were 3.2 seconds and 3.5 seconds, respectively. On average, the adenosine release starts and peaks 0.2 seconds prior to the oxygen. The A2a antagonist, SCH442416, decreased the number of both adenosine and oxygen transient events by about 32%. However, the A1 antagonist, DPCPX, did not significantly affect simultaneous adenosine and oxygen release. The nitric oxide (NO) synthase inhibitor L-NAME also did not affect the concentration or number of adenosine and oxygen release events. These results demonstrate that both adenosine and oxygen release are modulated via A2a receptors. The correlation of transient concentrations, time delay between adenosine and oxygen peaks, and effect of A2a receptors suggests adenosine modulates blood flow on a rapid, sub-second time scale.
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