Genome-Wide Identification of miRNAs and Their Targets Involved in the Developing Internodes under Maize Ears by Responding to Hormone Signaling.
Genome-Wide Identification of miRNAs and Their Targets Involved in the Developing Internodes under Maize Ears by Responding to Hormone Signaling.
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通过响应激素信号对参与玉米穗节间发育的 miRNA 及其靶标进行全基因组鉴定
DOI：
10.1371/journal.pone.0164026
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Tang J
中科院分区：
文献类型：
--
作者：
Zhao Z;Xue Y;Yang H;Li H;Sun G;Zhao X;Ding D;Tang J
Internode length is one of the decisive factors affecting plant height (PH) and ear height (EH), which are closely associated with the lodging resistance, biomass and grain yield of maize. miRNAs, currently recognized as important transcriptional/ post-transcriptional regulators, play an essential role in plant growth and development. However, their roles in developing internodes under maize ears remain unclear. To identify the roles of miRNAs and their targets in the development of internodes under maize ears, six miRNA and two degradome libraries were constructed using the 7th, 8th and 9th internodes of two inbred lines, ‘Xun928’ and ‘Xun9058’, which had significantly different internode lengths. A total of 45 and 54 miRNAs showed significant changes for each pairwise comparison among the 7th, 8th and 9th internodes of ‘Xun9058’ and ‘Xun928’, respectively. The expression of 31 miRNAs showed significant changes were common to the corresponding comparison groups of the 7th, 8th and 9th internodes of ‘Xun9058’ and ‘Xun928’. For the corresponding internodes of ‘Xun9058’ and ‘Xun928’, compared with the expression of miRNAs in the 7th, 8th and 9th internodes of ‘Xun928’, the numbers of up-regulated and down-regulated miRNAs were 11 and 36 in the 7th internode, 9 and 45 in the 8th internode, and 9 and 25 in the 9th internode of ‘Xun9058’, respectively. Moreover, 10 miRNA families containing 45 members showed significant changes at least in two internodes of ‘Xun928’ by comparing with the corresponding internodes of ‘Xun9058’. Based on the sequencing data, 20 miRNAs related to hormone signaling among the candidates, belonging to five conserved miRNA families, were selected for expression profiling using quantitative reverse-transcription polymerase chain reaction (qRT-PCR). The five miRNA families, zma-miR160, zma-miR167, zma-miR164, zma-miR169 and zma-miR393, targeted the genes encoding auxin response factor, N-acetylcysteine domain containing protein, nuclear transcription factor Y and auxin signaling F-BOX 2 through degradome sequencing. The miRNAs might regulate their targets to respond to hormone signaling, thereby regulating the internode elongation and development under maize ear. These results provide valuable reference for understanding the possible regulation mechanism of the ILs under the ear.
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DOI：
10.1073/pnas.85.23.9031
发表时间：
1988-12-01
影响因子：
11.1
作者：
FUJIOKA, S;YAMANE, H;TAKAHASHI, N
通讯作者：
TAKAHASHI, N
影响因子：
3.7
作者：
Ferreira TH;Gentile A;Vilela RD;Costa GG;Dias LI;Endres L;Menossi M
通讯作者：
Menossi M
影响因子：
3.7
作者：
Fahlgren N;Howell MD;Kasschau KD;Chapman EJ;Sullivan CM;Cumbie JS;Givan SA;Law TF;Grant SR;Dangl JL;Carrington JC
通讯作者：
Carrington JC
影响因子：
3.7
作者：
Leyva-González MA;Ibarra-Laclette E;Cruz-Ramírez A;Herrera-Estrella L
通讯作者：
Herrera-Estrella L
影响因子：
11.6
作者：
BENSEN, RJ;JOHAL, GS;BRIGGS, SP
通讯作者：
BRIGGS, SP