Effects of salivary protein flow and indigenous microorganisms on initial colonization of Candida albicans in an in vivo model.
Effects of salivary protein flow and indigenous microorganisms on initial colonization of Candida albicans in an in vivo model.
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唾液蛋白流量和土著微生物对体内模型中白色念珠菌初始定殖的影响。
DOI：
10.1186/1472-6831-12-36
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发表时间：
2012-08-31
期刊：
影响因子：
2.9
通讯作者：
Senpuku H
中科院分区：
文献类型：
--
作者：
Kanaguchi N;Narisawa N;Ito T;Kinoshita Y;Kusumoto Y;Shinozuka O;Senpuku H
白色念珠菌是一种二型性真菌，是口腔内微生物植物群的一部分。当宿主免疫防御功能受损或正常微生物植物群受到干扰时，C.白色念珠菌引发口腔粘膜和舌头的复发性感染。最近，我们生产了NOD/SCID.e2f1-/-小鼠，其显示出唾液过少、唾液蛋白流减少、唾液中缺乏伊加和IgG，并且具有减少的NK细胞。我们的目标是描述C。白念珠菌感染和生物膜形成。NOD/SCID.e2f1-/-小鼠用作C.白色念珠菌感染C.用洗必泰消毒后，将白色念珠菌酵母菌和菌丝体溶液引入口腔。C.接种后NOD/SCID.e2f1+/+中白念珠菌定殖并呈时间依赖性下降。然而，NOD/SCID.e2f1+/+小鼠的定植水平高于NOD/SCID.e2f1-/-小鼠。在接种前喂以1%蔗糖水的小鼠中，C.白色念珠菌样品被口腔中的固有微生物高度污染;而在未喂食蔗糖水的小鼠中没有。梭菌建群白色念珠菌不受土著微生物污染的影响。C.白色念珠菌限制了土著微生物的恢复。NOD/SCID.e2f1-/-组唾液减少并不增加C.白色念珠菌与NOD/SCID.e2f1+/+小鼠相比。提示唾液中C. NOD/SCID.e2f1-/-小鼠的口腔中可能无法充分提供白色念珠菌。唾液蛋白流对C.白色念珠菌初始定植，其中固有微生物不影响口腔中的定植。
Candida albicans is a dimorphic fungus that is part of the commensal microbial flora of the oral cavity. When the host immune defenses are impaired or when the normal microbial flora is disturbed, C. albicans triggers recurrent infections of the oral mucosa and tongue. Recently, we produced NOD/SCID.e2f1-/- mice that show hyposalivation, decrease of salivary protein flow, lack IgA and IgG in saliva, and have decreased NK cells. Our objective was to characterize C. albicans infection and biofilm formation in mice. NOD/SCID.e2f1-/- mice were used as an animal model for C. albicans infection. C. albicans yeast and hyphal forms solutions were introduced in the oral cavity after disinfection by Chlorhexidine. The numbers of C. albicans colonized and decreased in a time-dependent manner in NOD/SCID.e2f1+/+ after inoculation. However, the colonization levels were higher in NOD/SCID.e2f1+/+ than NOD/SCID.e2f1-/- mice. In the mice fed 1% sucrose water before inoculation, C. albicans sample was highly contaminated by indigenous microorganisms in the oral cavity; and was not in the mice fed no sucrose water. The colonization of C. albicans was not influenced by the contamination of indigenous microorganisms. The hyphal form of C. albicans restricted the restoration of indigenous microorganisms. The decreased saliva in NOD/SCID.e2f1-/- did not increase the colonization of C. albicans in comparison to NOD/SCID.e2f1+/+ mice. We suggest that the receptor in saliva to C. albicans may not be sufficiently provided in the oral cavity of NOD/SCID.e2f1-/- mice. The saliva protein flow may be very important for C. albicans initial colonization, where the indigenous microorganisms do not affect colonization in the oral cavity.
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影响因子：
3.2
作者：
Matsui-Inohara, Hikaru;Uematsu, Hiroshi;Senpuku, Hidenobu
通讯作者：
Senpuku, Hidenobu
影响因子：
3.2
作者：
MONTES, LF;WILBORN, WH
通讯作者：
WILBORN, WH
影响因子：
3.1
作者：
Fu, Y;Rieg, G;Filler, SG
通讯作者：
Filler, SG
影响因子：
4.9
作者：
Kamai, Y;Kubota, M;Filler, SG
通讯作者：
Filler, SG
DOI：
10.1016/0030-4220(94)90148-1
发表时间：
1994-08-01
影响因子：
--
作者：
CHALLACOMBE, SJ
通讯作者：
CHALLACOMBE, SJ