CRAM 3.1: advances in the CRAM file format.

CRAM 3.1: advances in the CRAM file format.
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DOI:
10.1093/bioinformatics/btac010
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发表时间:
2022-03-04
期刊:
Bioinformatics (Oxford, England)
影响因子:
--
通讯作者:
Bonfield JK
Bonfield JK
中科院分区:
其他
文献类型:
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作者:
Bonfield JK

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CRAM已经确立了自己作为一个高压缩替代BAM文件格式的DNA测序数据。我们描述了更新,以进一步改善这一现代测序仪器。使用Illumina数据,CRAM 3.1比等效的CRAM 3.0文件小7-15%,比相应的BAM文件小50-70%。由于存在高熵信号,长读技术显示出更适度的压缩。CRAM 3.0规范可从https://samtools.github.io/hts-specs/CRAMv3.pdf免费获得。CRAM 3.1的改进可以在https://github.com/samtools/htscodecs的一个单独的开源HTScodecs库中获得,并且已经被合并到HTSlib中。 补充数据可在Bioinformatics在线获得。
CRAM has established itself as a high compression alternative to the BAM file format for DNA sequencing data. We describe updates to further improve this on modern sequencing instruments. With Illumina data CRAM 3.1 is 7–15% smaller than the equivalent CRAM 3.0 file, and 50–70% smaller than the corresponding BAM file. Long-read technology shows more modest compression due to the presence of high-entropy signals. The CRAM 3.0 specification is freely available from https://samtools.github.io/hts-specs/CRAMv3.pdf. The CRAM 3.1 improvements are available in a separate OpenSource HTScodecs library from https://github.com/samtools/htscodecs, and have been incorporated into HTSlib. Supplementary data are available at Bioinformatics online.
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