Levels of Selenomonas species in generalized aggressive periodontitis.
Levels of Selenomonas species in generalized aggressive periodontitis.
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在广义攻击性牙周炎中的硒瘤物种水平。
DOI：
10.1111/j.1600-0765.2012.01485.x
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发表时间：
2012-12
影响因子：
3.5
通讯作者：
Faveri M
中科院分区：
文献类型：
--
作者：
Gonçalves LF;Fermiano D;Feres M;Figueiredo LC;Teles FR;Mayer MP;Faveri M
目的比较泛发性侵袭性牙周炎(GAgP)和牙周健康(PH)患者龈下生物膜中痰单胞菌和未培养/未识别的Selenomonas菌种的水平。收集GAgP(n=15)和PH(n=15)受试者的牙周临床参数。采集龈下菌斑样本(9个样本/受试者)，并使用RNA-寡核苷酸定量技术(ROQT)分析10个细菌分类群的水平，包括培养的和未培养的/未识别的微生物。使用Mann-Whitney检验来寻找不同组之间测试分类群水平的差异。GAgP组牙龈卟啉单胞菌、痰色单胞菌和Selenomonas口腔克隆CS002(人类口腔微生物数据库(HOMD)Oral Taxon 131)的平均计数显著高于PH组，而放线菌和血链球菌的平均计数高于PH组(p<；0.01)。硒单胞菌EW084(HOMD OT146)仅在GAgP组检出。在GAgP组中，牙周探诊深度(PD)≥为5 mm的部位牙龈假单胞菌和金黄色葡萄球菌的水平高于浅部(PD≤3 mm)(p<；0.0 1)。此外，Pd≤为3 mM的GAgP受试者比PH受试者拥有更高水平的这两种物种。PD与牙周炎假单胞菌(r=0.77；p<；0.01)、痰葡萄球菌(r=0.60；p<；0.01)和单胞菌呈正相关。EW076(OT 139)(r=042，p<；0.05)。Sputigena，Selenomonas sp.OracleCS002(OT131)和Selenomonas sp.口腔克隆EW084(OT146)可能与GAgP的发病机制有关，其在GAgP发生发展中的作用有待进一步研究。
To compare the levels of Selenomonas sputigena and uncultivated/unrecognized Selenomonas species in subgingival biofilms from generalized aggressive periodontitis (GAgP) and periodontaly healthy (PH) subjects. GAgP (n=15) and PH (n=15) subjects were recruited and their clinical periodontal parameters were evaluated. Subgingival plaque samples were collected (9 samples/subject) and analyzed for the levels of 10 bacterial taxa, including cultivated and uncultivated/unrecognized microorganisms using the RNA-oligonucleotide quantification technique (ROQT). Differences in the levels of the test taxa between groups were sought using the Mann-Whitney test. GAgP subjects showed significantly higher mean counts of Porphyromonas gingivalis, Selenomonas sputigena and Selenomonas oral clone CS002 (Human Oral Microbial Database (HOMD) Oral Taxon 131), while Actinomyces gerencseriae and Streptococcus sanguinis were found in higher mean counts in PH subjects (p<0.01). Selenomonas EW084 (HOMD OT 146) was only detected in the GAgP group. In the GAgP group, levels of P. gingivalis and S. sputigena were higher in sites with probing depth (PD) ≥5mm than in shallow sites (PD ≤3mm) (p<0.01). Furthermore, sites with PD≤3mm in GAgP subjects harbored higher levels of these two species than sites in PH subjects. There were positive correlations between PD and levels of P. gingivalis (r=0.77; p<0.01), S. sputigena (r=0.60; p<0.01) and Selenomonas sp. EW076 (OT 139) (r=042, p<0.05). S. sputigena, Selenomonas sp. oral CS002 (OT 131) and Selenomonas sp. oral clone EW084 (OT 146) may be associated with the pathogenesis of GAgP, and their role in the onset and progression of this infection should be further investigated.
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影响因子：
7.6
作者：
Kumar, PS;Griffen, AL;Leys, EJ
通讯作者：
Leys, EJ
影响因子：
6.7
作者：
Darby, IB;Hedge, PJ;Kinane, DF
通讯作者：
Kinane, DF
影响因子：
3.7
作者：
Kumar PS;Brooker MR;Dowd SE;Camerlengo T
通讯作者：
Camerlengo T
影响因子：
4.3
作者：
Haraszthy, VI;Hariharan, G;Zambon, JJ
通讯作者：
Zambon, JJ
影响因子：
9.4
作者：
Kumar, PS;Griffen, AL;Leys, EJ
通讯作者：
Leys, EJ