A minimally invasive rabbit model of progressive and reproducible disc degeneration confirmed by radiology, gene expression, and histology.
A minimally invasive rabbit model of progressive and reproducible disc degeneration confirmed by radiology, gene expression, and histology.
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DOI：
10.3340/jkns.2013.53.6.323
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发表时间：
2013-06
影响因子：
1.6
通讯作者：
Kwon YJ
中科院分区：
文献类型：
--
作者：
Kwon YJ
通过经皮环形穿刺建立一种简单、可重复的兔椎间盘退变模型，并确认退变程度随时间的变化。对15只新西兰白色家兔（4 - 5月龄，每只体重约3 - 3.5 kg）进行L2-L3、L3-L4和L4-L5椎间盘环形穿刺。在穿刺后4、8或20周处死家兔。对于评估椎间盘高度随时间变化的纵向研究，在0、2、4、8和20周时对20周组的家兔进行了连续X线检查。处死后，提取整个脊柱和椎间盘，并通过磁共振成像（MRI）、真实的时间逆转录聚合酶链反应和组织学染色进行分析。X线片显示椎间盘高度随时间缓慢、进行性降低。与术前椎间盘高度相比，在此期间观察到椎间盘间隙明显变窄（p<0.001）。MRI分级、聚集蛋白聚糖和基质金属蛋白酶-13 mRNA表达以及苏木精和伊红/番红O/抗胶原蛋白II染色一致指示变性，支持X线数据的结果。经皮环形穿刺导致缓慢的，可重复的椎间盘退变，这是由放射学，生物化学和组织学证实。该体内模型可用于研究和评价退行性椎间盘疾病生物治疗的安全性和有效性。
To develop a simple, reproducible model of disc degeneration in rabbits through percutaneous annular puncture and to confirm the degree of degeneration over time. Fifteen New Zealand white rabbits (4 to 5 months old and weighing approximately 3 to 3.5 kg each) underwent annular puncture of the L2-L3, L3-L4, and L4-L5 discs. Rabbits were sacrificed at 4, 8, or 20 weeks after puncture. For a longitudinal study to assess changes in disc height over time, serial X-rays were performed at 0, 2, 4, 8, and 20 weeks for rabbits in the 20-week group. Upon sacrifice, the whole spinal column and discs were extracted and analyzed with magnetic resonance imaging (MRI), real time reverse transcriptase-polymerase chain reaction, and histological staining. The X-rays showed a slow, progressive decrease in disc height over time. Significant disc space narrowing compared to preoperative disc height was observed during the time period (p<0.001). The MRI grade, aggrecan, and matrix metalloprotease-13 mRNA expression and hematoxylin and eosin/safranin O/anti-collagen II staining were consistently indicative of degeneration, supporting the results of the X-ray data. Percutaneous annular puncture resulted in slow, reproducible disc degeneration that was confirmed by radiology, biochemistry, and histology. This in vivo model can be used to study and evaluate the safety and efficacy of biologic treatments for degenerative disc disease.
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影响因子：
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