Leukocyte telomere dynamics across gestation in uncomplicated pregnancies and associations with stress.
Leukocyte telomere dynamics across gestation in uncomplicated pregnancies and associations with stress.
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DOI：
10.1186/s12884-022-04693-0
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发表时间：
2022-05-02
影响因子：
3.1
通讯作者：
Bianco, Katherine
中科院分区：
文献类型：
--
作者：
Panelli, Danielle M.;Leonard, Stephanie A.;Wong, Ronald J.;Becker, Martin;Mayo, Jonathan A.;Wu, Erica;Girsen, Anna, I;Gotlib, Ian H.;Aghaeepour, Nima;Druzin, Maurice L.;Shaw, Gary M.;Stevenson, David K.;Bianco, Katherine
短的白细胞端粒长度是一个生物标志物与压力和发病率在非怀孕的成年人。然而，对孕期母体端粒动力学知之甚少。为了解决这个问题，我们研究了无并发症妊娠期间母体白细胞端粒长度（LTL）的变化，并探讨了与感知压力的相关性。在这项试点研究中，从2012年至2018年在一家机构分娩活产足月单胎婴儿的产妇身上采集的血液中测量了产妇LTL。如果参与者患有糖尿病或高血压疾病，则被排除在外。在整个妊娠过程中收集样本，并将其分为三个时间段：< 200/7周（时间点1）；201/7至366/7周（时间点2）；产后370/7至9周（时间点3）。所有参与者还完成了一项调查，评估了在妊娠早期入学时感知压力的多变量概况。LTL采用定量聚合酶链反应（PCR）测定。使用Wilcoxon符号秩检验来比较参与者在所有时间点间隔内的LTL差异。为了确定分娩方式是否影响LTL，我们比较了阴道分娩和剖宫产分娩参与者的产后时间3点LTL。其次，我们使用机器学习分析评估了评估的多变量应力剖面和LTL之间的关联。共分析46例患者115份样本。以端粒与单拷贝基因（T/S）比表示的LTL （mean±SD）在时间点1、2和3分别为1.15±0.26、1.13±0.23和1.07±0.21。时间点1和时间点2的LTL无显著差异（LTL T/S变化- 0.03±0.26,p = 0.39）；2和3(−0.07±0.29,p = 0.38)或时间点1和3(−0.07±0.21,p = 0.06)。剖宫产组的产后LTLs明显短于顺产组（T/S比：剖宫产0.94±0.12 vs阴道1.12±0.21,p = 0.01）。在二次分析中，较差的睡眠质量是与较短的时间点1 LTLs （p = 0.02）和较短的平均LTLs （p = 0.03）相关的主要压力结构。在健康妊娠队列中，产妇ltl在整个妊娠期间没有显著变化，剖宫产后的产后ltl比顺产后短。睡眠质量与短睡眠时间之间的显著关联值得进一步研究。在线版本包含补充材料，可在10.1186/s12884-022-04693-0获得。
Short leukocyte telomere length is a biomarker associated with stress and morbidity in non-pregnant adults. Little is known, however, about maternal telomere dynamics in pregnancy. To address this, we examined changes in maternal leukocyte telomere length (LTL) during uncomplicated pregnancies and explored correlations with perceived stress. In this pilot study, maternal LTL was measured in blood collected from nulliparas who delivered live, term, singleton infants between 2012 and 2018 at a single institution. Participants were excluded if they had diabetes or hypertensive disease. Samples were collected over the course of pregnancy and divided into three time periods: < 200/7 weeks (Timepoint 1); 201/7 to 366/7 weeks (Timepoint 2); and 370/7 to 9-weeks postpartum (Timepoint 3). All participants also completed a survey assessing a multivariate profile of perceived stress at the time of enrollment in the first trimester. LTL was measured using quantitative polymerase chain reaction (PCR). Wilcoxon signed-rank tests were used to compare LTL differences within participants across all timepoint intervals. To determine whether mode of delivery affected LTL, we compared postpartum Timepoint 3 LTLs between participants who had vaginal versus cesarean birth. Secondarily, we evaluated the association of the assessed multivariate stress profile and LTL using machine learning analysis. A total of 115 samples from 46 patients were analyzed. LTL (mean ± SD), expressed as telomere to single copy gene (T/S) ratios, were: 1.15 ± 0.26, 1.13 ± 0.23, and 1.07 ± 0.21 for Timepoints 1, 2, and 3, respectively. There were no significant differences in LTL between Timepoints 1 and 2 (LTL T/S change − 0.03 ± 0.26, p = 0.39); 2 and 3 (− 0.07 ± 0.29, p = 0.38) or Timepoints 1 and 3 (− 0.07 ± 0.21, p = 0.06). Participants who underwent cesareans had significantly shorter postpartum LTLs than those who delivered vaginally (T/S ratio: 0.94 ± 0.12 cesarean versus 1.12 ± 0.21 vaginal, p = 0.01). In secondary analysis, poor sleep quality was the main stress construct associated with shorter Timepoint 1 LTLs (p = 0.02) and shorter mean LTLs (p = 0.03). In this cohort of healthy pregnancies, maternal LTLs did not significantly change across gestation and postpartum LTLs were shorter after cesarean than after vaginal birth. Significant associations between sleep quality and short LTLs warrant further investigation. The online version contains supplementary material available at 10.1186/s12884-022-04693-0.
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影响因子：
3.7
作者：
Liang G;Schernhammer E;Qi L;Gao X;De Vivo I;Han J
通讯作者：
Han J
影响因子：
2.2
作者：
Lin, Jue;Epel, Elissa;Cheon, Joshua;Kroenke, Candyce;Sinclair, Elizabeth;Bigos, Marty;Wolkowitz, Owen;Mellon, Synthia;Blackburn, Elizabeth
通讯作者：
Blackburn, Elizabeth
影响因子：
4.1
作者：
Lin, Jue;Cheon, Joshua;Blackburn, Elizabeth H.
通讯作者：
Blackburn, Elizabeth H.
影响因子：
3.7
作者：
Puterman E;Lin J;Blackburn E;O'Donovan A;Adler N;Epel E
通讯作者：
Epel E
影响因子：
4
作者：
Perales-Puchalt, Alfredo;Soberon, Nora;Blasco, Maria A.
通讯作者：
Blasco, Maria A.