Following de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage.
Following de novo triglyceride dynamics in ovaries of Aedes aegypti during the previtellogenic stage.
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DOI：
10.1038/s41598-021-89025-6
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发表时间：
2021-05-05
影响因子：
4.6
通讯作者：
Fernandez-Lima F
中科院分区：
文献类型：
--
作者：
Tose LV;Weisbrod CR;Michalkova V;Nouzova M;Noriega FG;Fernandez-Lima F
了解蚊子卵发育的分子和生化基础是蚊子生殖生物学研究的中心课题。脂质是能量的主要来源，也是卵泡发育的主要组成部分。超高分辨率质谱（UHRMS）结合用氘水（2H2O）进行卵泡脂质的体内代谢标记，可以明确鉴定卵巢发育过程中新生的脂质种类。在本研究中，我们对雌性埃及伊蚊成虫卵巢卵黄发生前（PVG）阶段（羽化后2-7天）的新生甘油三酯（TG）动态进行了研究。采用液相色谱（LC）和配备六极检测的14.5 T傅立叶变换离子回旋共振质谱仪（14.5 T FT-ICR MS）的高精度（< 0.3 ppm）和高质量分辨率（超过1 M），对饲料中稳定同位素的掺入进行了评估。LC-UHRMS提供了有效的基于同位素精细结构的脂类分离和化学式鉴定。监测稳定同位素掺入到新生掺入的tg中，表明卵巢脂质在PVG阶段被消耗或再循环，具有可变的时间动态。这些结果进一步证明了蚊子卵发生过程中卵泡脂质动力学的分子机制的复杂性。
Understanding the molecular and biochemical basis of egg development is a central topic in mosquito reproductive biology. Lipids are a major source of energy and building blocks for the developing ovarian follicles. Ultra-High Resolution Mass Spectrometry (UHRMS) combined with in vivo metabolic labeling of follicle lipids with deuterated water (2H2O) can provide unequivocal identification of de novo lipid species during ovarian development. In the present study, we followed de novo triglyceride (TG) dynamics during the ovarian previtellogenic (PVG) stage (2–7 days post-eclosion) of female adult Aedes aegypti. The incorporation of stable isotopes from the diet was evaluated using liquid chromatography (LC) in tandem with the high accuracy (< 0.3 ppm) and high mass resolution (over 1 M) of a 14.5 T Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (14.5 T FT-ICR MS) equipped with hexapolar detection. LC-UHRMS provides effective lipid class separation and chemical formula identification based on the isotopic fine structure. The monitoring of stable isotope incorporation into de novo incorporated TGs suggests that ovarian lipids are consumed or recycled during the PVG stage, with variable time dynamics. These results provide further evidence of the complexity of the molecular mechanism of follicular lipid dynamics during oogenesis in mosquitoes.
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影响因子：
7.4
作者：
Benigni P;Thompson CJ;Ridgeway ME;Park MA;Fernandez-Lima F
通讯作者：
Fernandez-Lima F
影响因子：
7.4
作者：
Cho, Eunji;Witt, Matthias;Kim, Sunghwan
通讯作者：
Kim, Sunghwan
DOI：
10.1007/s12127-016-0198-z
发表时间：
2016-09-01
影响因子：
--
作者：
Adams, Kendra J.;Montero, Dennise;Fernandez-Lima, Francisco
通讯作者：
Fernandez-Lima, Francisco
影响因子：
56.9
作者：
CHINO, H;GILBERT, LI
通讯作者：
GILBERT, LI
影响因子：
3.9
作者：
Hill, S;Winning, B;Leaver, C
通讯作者：
Leaver, C