Impact of Different Durations of Fasting on Intestinal Autophagy and Serum Metabolome in Broiler Chicken.
Impact of Different Durations of Fasting on Intestinal Autophagy and Serum Metabolome in Broiler Chicken.
复制标题
不同禁食时间对肉鸡肠道自噬和血清代谢组的影响
DOI：
10.3390/ani11082183
复制
发表时间：
2021-07-23
期刊：
影响因子：
--
通讯作者：
Yuan J
中科院分区：
文献类型：
--
作者：
Wang Y;Xu Y;Wu Y;Mahmood T;Chen J;Guo X;Wu W;Wang B;Guo Y;Yuan J
在家禽代谢能评估之前，通常使用禁食。近年来，由于自噬的有益功能，禁食诱导的自噬受到关注。在这项研究中，我们发现肠道自噬基因Atg 7与禁食时间有很好的二次拟合。我们发现，甘油磷脂、苯丙氨酸、GnRH信号通路、糖基磷脂酰肌醇锚生物合成、自噬和铁凋亡等血清代谢途径随禁食而改变。此外，我们发现肠自噬与血清代谢产物PE之间存在相关性（18：3（9 Z，12 Z，15 Z）/P-18：0）。摘要禁食诱导的肠道自噬对机体健康有益。本研究旨在探讨宿主代谢与肠道自噬的关系。将肉鸡随机分配到48个笼中。在第25天上午09：00前0（CT）、12（FH 12）、24（FH 24）、36（FH 36）、48（FH 48）和72 h（FH 72），采用完全随机设计将8笼鸡随机分配到每个禁食时间点，并将其食物取出。在第25天上午09：00，分别采集血液和空肠样品用于血清代谢组学和自噬基因分析。结果表明，自噬基因Atg 7与禁食时间有良好的二次拟合关系（R2 = 0.432，p < 0.001）。禁食24 h或更长时间的鸡血清磷脂酰乙醇胺（PE）和溶血PE降低。相反，血清中的磷脂酰胆碱（PC）和溶血PC增加的鸟类禁食36小时或更长时间。代谢途径分析显示，随着禁食时间的延长，血清甘油磷脂、苯丙氨酸和GnRH信号通路下调。参与糖基磷脂酰肌醇锚生物合成、自噬和铁凋亡的血清代谢产物在所有禁食组中均上调。相关分析显示，血清PE（18：3（9 Z，12 Z，15 Z）/P-18：0）是肠自噬的潜在生物标志物。我们的研究结果提供了一个潜在的生物标志物相关的肠自噬。
Simple Summary Fasting is usually used before metabolizable energy assessment in poultry. Recently, fasting-induced autophagy has been of concern because of the beneficial function of autophagy. In this study, we found that the intestinal autophagy gene Atg7 has a good quadratic fitting with fasting duration. We found that the serum metabolism pathways involved in glycerophospholipid, phenylalanine, GnRH signaling pathways, glycosylphosphatidylinositol anchor biosynthesis, autophagy, and ferroptosis changed with fasting. Furthermore, we found a correlation between intestinal autophagy and serum metabolite PE (18:3(9Z,12Z,15Z)/P-18:0). Abstract Fasting-induced autophagy in the intestine is beneficial for body health. This study was designed to explore the relationship between the host metabolism and intestinal autophagy. Broilers were randomly assigned into 48 cages. At 0 (CT), 12 (FH12), 24 (FH24), 36 (FH36), 48(FH48), and 72 h (FH72) before 09:00 a.m. on day 25, eight cages of birds were randomly allotted to each fasting time point using completely random design, and their food was removed. At 09:00 a.m. on day 25, the blood and jejunum were sampled for serum metabolome and autophagy gene analyses, respectively. The results showed that the autophagy gene Atg7 has a good quadratic fit with fasting duration (R2 = 0.432, p < 0.001). Serum phosphatidylethanolamine (PE) and lyso-PE were decreased in the birds that were fasted for 24 h or longer. Conversely, the serum phosphatidylcholine (PC) and lyso-PC were increased in the birds that were fasted for 36 h or longer. Metabolism pathway analysis showed that the serum glycerophospholipid, phenylalanine, and GnRH signaling pathways were downregulated with the extended fasting duration. The serum metabolites involved in glycosylphosphatidylinositol anchor biosynthesis, autophagy, and ferroptosis were upregulated in all of the fasted groups. Correlation analysis showed that serum PE (18:3(9Z,12Z,15Z)/P-18:0) was a potential biomarker for intestinal autophagy. Our findings provide a potential biomarker related to intestinal autophagy.
登录
查看更多内容
ACSL4 通过塑造细胞脂质成分来决定铁死亡敏感性。
影响因子：
14.8
作者：
Doll S;Proneth B;Tyurina YY;Panzilius E;Kobayashi S;Ingold I;Irmler M;Beckers J;Aichler M;Walch A;Prokisch H;Trümbach D;Mao G;Qu F;Bayir H;Füllekrug J;Scheel CH;Wurst W;Schick JA;Kagan VE;Angeli JP;Conrad M
通讯作者：
Conrad M
影响因子：
14.8
作者：
Kagan VE;Mao G;Qu F;Angeli JP;Doll S;Croix CS;Dar HH;Liu B;Tyurin VA;Ritov VB;Kapralov AA;Amoscato AA;Jiang J;Anthonymuthu T;Mohammadyani D;Yang Q;Proneth B;Klein-Seetharaman J;Watkins S;Bahar I;Greenberger J;Mallampalli RK;Stockwell BR;Tyurina YY;Conrad M;Bayır H
通讯作者：
Bayır H
影响因子：
4.6
作者：
Dashti, Monireh;Kulik, Willem;Hoek, Frans;Veerman, Enno C.;Peppelenbosch, Maikel P.;Rezaee, Farhad
通讯作者：
Rezaee, Farhad
影响因子：
3.6
作者：
Lu, Zhuang;He, Xiaofang;Gao, Feng
通讯作者：
Gao, Feng
DOI：
10.1097/00005176-198611000-00009
发表时间：
1986-11-01
影响因子：
2.9
作者：
ARISAKA, M;ARISAKA, O;YAMASHIRO, Y
通讯作者：
YAMASHIRO, Y