Ultrahigh speed spectral / Fourier domain OCT ophthalmic imaging at 70,000 to 312,500 axial scans per second.
Ultrahigh speed spectral / Fourier domain OCT ophthalmic imaging at 70,000 to 312,500 axial scans per second.
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DOI：
10.1364/oe.16.015149
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发表时间：
2008-09-15
期刊：
影响因子：
3.8
通讯作者：
Fujimoto JG
中科院分区：
文献类型：
--
作者：
Potsaid B;Gorczynska I;Srinivasan VJ;Chen Y;Jiang J;Cable A;Fujimoto JG
我们展示了1000 - 312，500轴向扫描每秒的速率下使用1000 - 312，500的CMOS线扫描相机的1000 - 1000速度的光谱/傅立叶域光学相干断层扫描（OCT）。对几种设计配置进行了表征，以说明采集速度、分辨率、成像范围、灵敏度和灵敏度滚降性能之间的权衡。具有2.5 - 3.0微米轴向图像分辨率的超高分辨率OCT被证明在每秒100，000次轴向扫描下。高分辨率光谱仪设计提高了灵敏度滚降和成像范围性能，将成像速度降低到每秒70，000次轴向扫描。还展示了每秒> 300，000次轴向扫描的超高速成像以及标准图像分辨率。研究了正常人视网膜的眼科OCT成像。高采集速度使得密集光栅扫描能够以最小的运动伪影采集黄斑和视盘的密集采样的体积三维OCT（3D-OCT）数据集。在每秒250，000次轴向扫描的情况下，以1.8 - 9微米的轴向分辨率成像，仅需1.3秒即可获得512 × 512 × 400体素体积3D-OCT数据集。正交配准扫描用于配准OCT光栅扫描并去除残留的轴向眼睛运动，从而产生保留视网膜地形图的3D-OCT数据集。在小体积上的快速重复成像可以使小的视网膜特征可视化而没有运动引起的失真，并且使得体积配准能够消除眼睛运动。视网膜的某些区域中的视锥光感受器可以在没有自适应光学器件或主动眼跟踪的情况下可视化。3D体积的快速重复成像还提供动态体积信息（4D-OCT），其被示出为增强视网膜毛细血管的可视化并且应该能够实现功能成像。3D-OCT体积成像速度和性能的改进有望实现早期诊断和改善对眼科疾病进展和治疗反应的监测，并在其他领域具有广泛的研究和临床应用。
We demonstrate ultrahigh speed spectral / Fourier domain optical coherence tomography (OCT) using an ultrahigh speed CMOS line scan camera at rates of 70,000 - 312,500 axial scans per second. Several design configurations are characterized to illustrate trade-offs between acquisition speed, resolution, imaging range, sensitivity and sensitivity roll-off performance. Ultrahigh resolution OCT with 2.5 - 3.0 micron axial image resolution is demonstrated at ∼ 100,000 axial scans per second. A high resolution spectrometer design improves sensitivity roll-off and imaging range performance, trading off imaging speed to 70,000 axial scans per second. Ultrahigh speed imaging at >300,000 axial scans per second with standard image resolution is also demonstrated. Ophthalmic OCT imaging of the normal human retina is investigated. The high acquisition speeds enable dense raster scanning to acquire densely sampled volumetric three dimensional OCT (3D-OCT) data sets of the macula and optic disc with minimal motion artifacts. Imaging with ∼ 8 - 9 micron axial resolution at 250,000 axial scans per second, a 512 × 512 × 400 voxel volumetric 3D-OCT data set can be acquired in only ∼ 1.3 seconds. Orthogonal registration scans are used to register OCT raster scans and remove residual axial eye motion, resulting in 3D-OCT data sets which preserve retinal topography. Rapid repetitive imaging over small volumes can visualize small retinal features without motion induced distortions and enables volume registration to remove eye motion. Cone photoreceptors in some regions of the retina can be visualized without adaptive optics or active eye tracking. Rapid repetitive imaging of 3D volumes also provides dynamic volumetric information (4D-OCT) which is shown to enhance visualization of retinal capillaries and should enable functional imaging. Improvements in the speed and performance of 3D-OCT volumetric imaging promise to enable earlier diagnosis and improved monitoring of disease progression and response to therapy in ophthalmology, as well as have a wide range of research and clinical applications in other areas.
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