Differential Immunoreactivity to Bovine Convalescent Serum Between Mycoplasma bovis Biofilms and Planktonic Cells Revealed by Comparative Immunoproteomic Analysis.
Differential Immunoreactivity to Bovine Convalescent Serum Between Mycoplasma bovis Biofilms and Planktonic Cells Revealed by Comparative Immunoproteomic Analysis.
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DOI：
10.3389/fmicb.2018.00379
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发表时间：
2018
影响因子：
5.2
通讯作者：
Chu Y
中科院分区：
文献类型：
--
作者：
Chen S;Hao H;Zhao P;Ji W;Li M;Liu Y;Chu Y
牛支原体（Mycoplasma bovis）是一种主要的牛病原体，在世界范围内给养牛业造成巨大的经济损失。此外，M.牛的生物膜可以在环境和其宿主中持续存在。到目前为止，M。牛恢复期血清识别的牛生物膜抗原及其与嗜酸性细胞的比较尚未被探索。本研究利用免疫蛋白质组学方法，使用二维电泳，免疫印迹使用恢复期牛血清，和随后的基质辅助激光解吸/电离飞行时间串联质谱（MALDI-TOF/TOF MS），以确定表达的免疫反应性蛋白在生物膜和等离子体生长的M。牛08 M.结果表明，M.牛的生物膜和牙周膜细胞首次显示出对牛恢复期血清的不同免疫反应性。共鉴定了10个和8个免疫反应蛋白的生物膜和细胞，分别。据我们所知，在15种蛋白质中，有12种在M. bovis，6株为M.牛生物膜三种蛋白质，即，内切葡聚糖酶，巯基过氧化物酶，和一个假定的膜蛋白，即支原体免疫原性脂肪酶A，被确定在支原体细胞和生物膜。所鉴定的蛋白质大部分是主要参与运输和代谢的细胞质蛋白。ATP结合、氧化还原酶活性和GTP结合是其最具代表性的分子功能。DnaK和Tuf似乎是最具相互作用的免疫反应剂中确定的蛋白质。此外，6个蛋白质有潜力作为血清学诊断抗原。这些数据将有助于我们进一步了解寄主对M.牛的生物膜和增殖细胞，这可能有助于开发新的分子候选人的改进的诊断和疫苗，以防止M。牛传染病
Mycoplasma bovis is a major bovine pathogen that causes considerable economic losses in the cattle industry worldwide. Moreover, M. bovis biofilm can persist in the environment and its host. To date, M. bovis biofilm antigens recognized by bovine convalescent sera and their comparison with planktonic cells have not yet been explored. This study utilized an immunoproteomic approach using two-dimensional electrophoresis, immunoblotting using convalescent bovine serum, and subsequent matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS) to identify the immunoreactive proteins expressed in biofilm- and planktonic-grown M. bovis strain 08M. Results showed that M. bovis biofilms and planktonic cells demonstrate differential immunoreactivity to bovine convalescent serum for the first time. A total of 10 and 8 immunoreactive proteins were identified for biofilms and planktonic cells, respectively. To our knowledge, a total of 12 out of 15 had not been reported as immunoreactive proteins in M. bovis, and six were specific to M. bovis biofilms. Three proteins, namely, endoglucanase, thiol peroxidase, and one putative membrane protein, that is, mycoplasma immunogenic lipase A, were identified in planktonic cells and biofilms. Most of the identified proteins were cytoplasmic proteins that were mainly involved in transport and metabolism. Moreover, ATP binding, oxidoreductase activity, and GTP binding were their most representative molecular functions. DnaK and Tuf appeared to be the most interactive immunoreactive agent among the identified proteins. Furthermore, six proteins had potential as serodiagnostic antigens. These data will be helpful to improve our current understanding on the host response to M. bovis biofilms and planktonic cells, which may facilitate the development of novel molecular candidates of improved diagnostics and vaccines to prevent M. bovis infections.
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影响因子：
3.2
作者：
Kalmokoff, Martin;Lanthier, Patricia;Szymanski, Christine M.
通讯作者：
Szymanski, Christine M.
影响因子：
3.3
作者：
Robino, P;Alberti, A;Rosati, S
通讯作者：
Rosati, S
影响因子：
3.6
作者：
Carvalhais, Virginia;Cerveira, Frederico;Vitorino, Rui
通讯作者：
Vitorino, Rui
影响因子：
1.8
作者：
Jores, Joerg;Meens, Jochen;Gerlach, Gerald F.
通讯作者：
Gerlach, Gerald F.
影响因子：
3.3
作者：
SACHSE, K;PFUTZNER, H;HANEL, I
通讯作者：
HANEL, I