Phenotyping of left and right ventricular function in mouse models of compensated hypertrophy and heart failure with cardiac MRI.
Phenotyping of left and right ventricular function in mouse models of compensated hypertrophy and heart failure with cardiac MRI.
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DOI：
10.1371/journal.pone.0055424
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Nicolay K
中科院分区：
文献类型：
--
作者：
van Nierop BJ;van Assen HC;van Deel ED;Niesen LB;Duncker DJ;Strijkers GJ;Nicolay K
左心室（LV）和右心室（RV）功能对压力超负荷诱导的心力衰竭的症状发生、疾病进展和运动耐量有重要影响，但在相关主动脉结扎小鼠模型中，特别是RV功能变化没有得到很好的描述。因此，我们定量的时间依赖性改变的心室形态和功能在两个模型的肥厚和心力衰竭，我们研究了RV和LV功能之间的关系，从肥厚到心力衰竭的过渡。MRI用于量化健康（n = 4）和假手术（n = 3）C57 BL/6小鼠以及具有轻度（n = 5）和重度主动脉收缩（n = 10）的动物中的RV和LV功能和形态。 与对照组相比，轻度收缩的小鼠显示LV质量增加（P<0.01）和LV射血分数（EF）降低（P<0.05），但RVEF相似（P>0.05）。在10周的随访中，与对照组相比，严重收缩的动物逐渐发展为LV肥大（P <0.001），LVEF降低（P <0.001），随后是RVEF下降（P<0.001）和肺重塑的发展。与对照组相比，重度收缩组小鼠的心肌应变降低（P<0.05）。主动脉缩窄后小鼠RV和LV功能的相关变化可以使用MRI定量。本文描述的良好对照模型为评估新的MRI技术诊断心力衰竭的附加值和研究新的治疗策略对疾病进展和症状发生的影响提供了机会。
Left ventricular (LV) and right ventricular (RV) function have an important impact on symptom occurrence, disease progression and exercise tolerance in pressure overload-induced heart failure, but particularly RV functional changes are not well described in the relevant aortic banding mouse model. Therefore, we quantified time-dependent alterations in the ventricular morphology and function in two models of hypertrophy and heart failure and we studied the relationship between RV and LV function during the transition from hypertrophy to heart failure. MRI was used to quantify RV and LV function and morphology in healthy (n = 4) and sham operated (n = 3) C57BL/6 mice, and animals with a mild (n = 5) and a severe aortic constriction (n = 10). Mice subjected to a mild constriction showed increased LV mass (P<0.01) and depressed LV ejection fraction (EF) (P<0.05) as compared to controls, but had similar RVEF (P>0.05). Animals with a severe constriction progressively developed LV hypertrophy (P<0.001), depressed LVEF (P<0.001), followed by a declining RVEF (P<0.001) and the development of pulmonary remodeling, as compared to controls during a 10-week follow-up. Myocardial strain, as a measure for local cardiac function, decreased in mice with a severe constriction compared to controls (P<0.05). Relevant changes in mouse RV and LV function following an aortic constriction could be quantified using MRI. The well-controlled models described here open opportunities to assess the added value of new MRI techniques for the diagnosis of heart failure and to study the impact of new therapeutic strategies on disease progression and symptom occurrence.
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