Organising the cell cycle in the absence of transcriptional control: Dynamic phosphorylation co-ordinates the Trypanosoma brucei cell cycle post-transcriptionally
Organising the cell cycle in the absence of transcriptional control: Dynamic phosphorylation co-ordinates the Trypanosoma brucei cell cycle post-transcriptionally
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在缺乏转录控制的情况下组织细胞周期：动态磷酸化在转录后协调布氏锥虫细胞周期
DOI：
10.1101/615351
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Benz C
中科院分区：
文献类型：
--
作者：
Benz C
单细胞真核生物布鲁氏锥虫的细胞分裂周期受到严格调控，尽管缺乏转录调控，这是由于基因在多顺反子单元中的排列和缺乏动态调节的转录因子。为了确定动态磷酸化对布鲁氏锥虫细胞周期控制的贡献，我们通过离心洗脱和定量磷酸蛋白质组分析相结合的方式实现了细胞周期同步。细胞周期调节的磷酸化位点丰度变化(917个位点，平均变化5倍)比蛋白质丰度变化(443个蛋白质，平均变化2倍)更广泛，幅度更大，而且大多相互独立。根据细胞周期分布对共同调节的磷酸化位点进行的等级聚类表明，磷酸化在整个细胞周期中大量增加，已知的细胞周期调节因子和RNA结合蛋白(RBPs)在最大的簇中显著丰富。细胞周期调控的基本细胞周期蛋白激酶的变化在时间上与动粒和真核启动因子成分的差异磷酸化以及许多以前与细胞周期无关的限制性商业惯例相协调，例如含有8个PSP1-C末端结构域的蛋白质。时间分布证明了动态磷酸化在协调细胞周期进展中的重要性，并提供了限制性商业惯例在布鲁氏锥虫细胞周期的转录后调控中发挥核心作用的证据。数据可通过标识为PXD013488的ProteomeXchange获得。
The cell division cycle of the unicellular eukaryoteTrypanosome bruceiis tightly regulated despite the paucity of transcriptional control that results from the arrangement of genes in polycistronic units and lack of dynamically regulated transcription factors. To identify the contribution of dynamic phosphorylation toT.bruceicell cycle control we have combined cell cycle synchronisation by centrifugal elutriation with quantitative phosphoproteomic analysis. Cell cycle regulated changes in phosphorylation site abundance (917 sites, average 5-fold change) were more widespread and of a larger magnitude than changes in protein abundance (443 proteins, average 2-fold change) and were mostly independent of each other. Hierarchical clustering of co-regulated phosphorylation sites according to their cell cycle profile revealed that a bulk increase in phosphorylation occurs across the cell cycle, with a significant enrichment of known cell cycle regulators and RNA binding proteins (RBPs) within the largest clusters. Cell cycle regulated changes in essential cell cycle kinases are temporally co-ordinated with differential phosphorylation of components of the kinetochore and eukaryotic initiation factors, along with many RBPs not previously linked to the cell cycle such as eight PSP1-C terminal domain containing proteins. The temporal profiles demonstrate the importance of dynamic phosphorylation in co-ordinating progression through the cell cycle, and provide evidence that RBPs play a central role in post-transcriptional regulation of theT.bruceicell cycle.Data are available via ProteomeXchange with identifier PXD013488.
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影响因子：
4.4
作者：
Urbaniak MD;Martin DM;Ferguson MA
通讯作者：
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Urbaniak MD;Guther ML;Ferguson MA
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Erben ED;Fadda A;Lueong S;Hoheisel JD;Clayton C
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影响因子：
4
作者：
S. Pasion;G. W. Brown;Lisa M. Brown;D. Ray
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影响因子：
3.7
作者：
G. Levy;Carolina P. Bañuelos;Analía G Níttolo;Gastón E Ortiz;Nicolás Mendiondo;G. Moretti;V. Tekiel;D. Sánchez
通讯作者：
D. Sánchez