Vitamin A up-regulates expression of bone-type alkaline phosphatase in rat small intestinal crypt cell line and fetal rat small intestine.
Vitamin A up-regulates expression of bone-type alkaline phosphatase in rat small intestinal crypt cell line and fetal rat small intestine.
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维生素 A 上调大鼠小肠隐窝细胞系和胎鼠小肠中骨型碱性磷酸酶的表达。
DOI：
10.1093/jn/128.11.1869
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
K. Kishi
中科院分区：
文献类型：
--
作者：
T. Nikawa;K. Rokutan;K. Nanba;K. Tokuoka;S. Teshima;M. Engle;D. Alpers;K. Kishi
维生素A是多种组织中肝/骨/肾碱性磷酸酶（L/B/K ALP）的有效诱导剂。然而，维生素A在小肠中诱导L/B/K ALP的证据有限。本实验研究了维生素A对大鼠小肠隐窝IEC-6细胞和胎鼠小肠L/B/K ALP表达的影响。用全反式维甲酸（RA）处理IEC-6细胞可提高L/B/K ALP的活性、蛋白水平和mRNA水平，而小肠细胞特异性蛋白，包括肠ALP、蔗糖-异麦芽糖酶和葡萄糖转运蛋白-2未被诱导。逆转录聚合酶链反应技术显示，该L/B/K ALP转录物具有骨型而不具有肝型先导外显子。IEC-6细胞在不同浓度下组成性地表达维甲酸受体（RAR）和维甲酸X受体（RXR）所有亚型的mrna。在这些受体mRNA中，rrbeta mRNA对RA治疗反应迅速，在4小时内水平增加一倍。凝胶迁移量转移实验表明，RA在IEC-6细胞中诱导了rxre结合活性。在胎鼠小肠中表达的L/B/K ALP转录本也含有骨型领导外显子。妊娠第7 ~ 15天给孕鼠灌胃10 mg醋酸视黄酯可增加15天胎鼠小肠中该转录物和酶的水平。我们的研究结果表明维生素A可能是胎鼠小肠和IEC-6细胞中L/B/K ALP表达的重要调节因子。
Vitamin A is a potent inducer for liver/bone/kidney alkaline phosphatase (L/B/K ALP) in a variety of tissues. However, the evidence for induction of L/B/K ALP by vitamin A in small intestine is limited. In this study, we investigated the influence of vitamin A on L/B/K ALP expression in rat small intestinal crypt IEC-6 cells and fetal rat small intestine. Treatment of IEC-6 cells with all-trans retinoic acid (RA) increased the levels of activity, protein and mRNA of L/B/K ALP, whereas enterocyte-specific proteins, including intestinal ALP, sucrase-isomaltase and glucose transporter-2, were not induced. The reverse transcription-polymerase chain reaction technique revealed that this L/B/K ALP transcript had the bone-type but not the liver-type leader exon. IEC-6 cells constitutively expressed mRNAs of all subtypes of retinoic acid receptor (RAR) and retinoid X receptor (RXR) at varied concentrations. Among these receptor mRNAs, RARbeta mRNA quickly responded to RA treatment, and the level was doubled within 4 h. Gel mobility shift assay showed that RA induced an RXRE-binding activity in IEC-6 cells. The L/B/K ALP transcript, expressed in fetal rat small intestine, also contained the bone-type leader exon. Intragastric administration of 10 mg retinyl acetate to pregnant rats from gestational d 7 to 15 increased the levels of this transcript and enzyme in 15-d fetal rat small intestine. Our results suggest that vitamin A may be an important regulator for L/B/K ALP expression in fetal rat small intestine as well as in IEC-6 cells.
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肠道、肝脏和胎盘碱性磷酸酶的个体发生和系统发育研究。
影响因子：
29.4
作者：
Komoda,T;Koyama,I;Nagata,A;Sakagishi,Y;DeSchryver-Kecskemeti,K;Alpers,DH
通讯作者：
Alpers,DH
来自大鼠骨/肝/肾/胎盘基因的替代启动子的碱性磷酸酶的组织特异性和地塞米松诱导表达。
DOI：
10.1016/0006-291x(91)90405-v
发表时间：
1991
影响因子：
3.1
作者：
Zernik,J;Kream,B;Twarog,K
通讯作者：
Twarog,K
大鼠小肠隐窝-绒毛轴蔗糖酶-异麦芽糖酶基因表达的调节。
DOI：
10.1016/s0006-291x(05)80853-8
发表时间：
1990
影响因子：
3.1
作者：
Traber,PG
通讯作者：
Traber,PG
DOI：
10.1016/0006-291x(87)91481-1
发表时间：
1987-02-13
影响因子：
3.1
作者：
KUROKOWA, M;LYNCH, K;PODOLSKY, DK
通讯作者：
PODOLSKY, DK