Outer membrane vesicles of Helicobacter pylori TK1402 are involved in biofilm formation.
Outer membrane vesicles of Helicobacter pylori TK1402 are involved in biofilm formation.
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DOI：
10.1186/1471-2180-9-197
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发表时间：
2009-09-15
期刊：
影响因子：
4.2
通讯作者：
Kamiya S
中科院分区：
文献类型：
--
作者：
Yonezawa H;Osaki T;Kurata S;Fukuda M;Kawakami H;Ochiai K;Hanawa T;Kamiya S
幽门螺杆菌在体外分批培养中在空气-液体界面的玻璃表面上形成生物膜; pylori还没有被很好地描述。本研究分析了H. pylori菌株形成生物膜，并表征了H.幽门生物膜形成。菌株TK 1402在添加7%FCS的布鲁氏菌肉汤中显示出较强的生物膜形成能力。TK 1402较强的挂膜能力反映在生物膜的相对厚度上。此外，仅在TK 1402生物膜的基质中检测到外膜囊泡（OMV）。生物膜的形成与OMV的产生密切相关。我们进一步观察到菌株TK 1402在添加0.2% β-环糊精的布鲁氏菌肉汤中不形成厚的生物膜。然而，添加从TK 1402收集的OMV级分可以增强生物膜形成。结果表明，TK 1402产生的OMV在TK 1402的生物膜形成中起重要作用。
Helicobacter pylori forms biofilms on glass surfaces at the air-liquid interface in in vitro batch cultures; however, biofilms of H. pylori have not been well characterized. In the present study, we analyzed the ability of H. pylori strains to form biofilms and characterized the underlying mechanisms of H. pylori biofilm formation. Strain TK1402 showed strong biofilm forming ability relative to the other strains in Brucella broth supplemented with 7% FCS. The strong biofilm forming ability of TK1402 is reflected the relative thickness of the biofilms. In addition, outer membrane vesicles (OMV) were detected within the matrix of only the TK1402 biofilms. Biofilm formation was strongly correlated with the production of OMV in this strain. We further observed that strain TK1402 did not form thick biofilms in Brucella broth supplemented with 0.2% β-cyclodextrin. However, the addition of the OMV-fraction collected from TK1402 could enhance biofilm formation. The results suggested that OMV produced from TK1402 play an important role in biofilm formation in strain TK1402.
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DOI：
10.1128/cdli.12.2.347-353.2005
发表时间：
2005-02-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Nakagawa, S;Osaki, T;Kamiya, S
通讯作者：
Kamiya, S
影响因子：
9.4
作者：
Osaki, T;Taguchi, H;Kamiya, S
通讯作者：
Kamiya, S
影响因子：
6.4
作者：
Rupp, ME;Fey, PD;Götz, F
通讯作者：
Götz, F
DOI：
10.1073/pnas.93.25.14648
发表时间：
1996-12-10
影响因子：
11.1
作者：
Censini, S;Lange, C;Covacci, A
通讯作者：
Covacci, A
影响因子：
3.2
作者：
Danese, PN;Pratt, LA;Kolter, R
通讯作者：
Kolter, R