HPS4/SABRE regulates plant responses to phosphate starvation through antagonistic interaction with ethylene signalling.
HPS4/SABRE regulates plant responses to phosphate starvation through antagonistic interaction with ethylene signalling.
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HPS4/SABRE 通过与乙烯信号传导的拮抗相互作用调节植物对磷酸盐饥饿的反应
DOI：
10.1093/jxb/ers131
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发表时间：
2012-07
影响因子：
6.9
通讯作者：
Liu D
中科院分区：
文献类型：
--
作者：
Yu H;Luo N;Sun L;Liu D
多不饱和脂肪酸（PUFAs）是多种动物离子通道的强力调节剂。研究表明，PUFAs强烈影响植物TPC1基因编码的慢液泡通道（SV）的活性。采用膜片钳技术对胡萝卜主根和拟南芥叶肉细胞的空泡进行分离，选择花生四烯酸（AA）作为PUFAs的模型分子。我们的研究扩展到不同的PUFAs，包括内源性α -亚麻酸（ALA）。微摩尔浓度AA的加入可逆地抑制了SV通道，降低了SV通道的最大打开概率，使半激活电压变为正值。比较不同PUFAs对通道调制的影响，发现亲脂酰基链的长度、双键数和极性头是通道调制的关键因素。实验数据可以通过一个简单的三态模型重现，其中pufa不直接与电压传感器相互作用，而是影响电压无关的转换，导致通道从打开状态到关闭配置。结果表明，脂质在协调离子通道活动中起着重要作用，类似于从动物细胞中已知的作用。
Polyunsaturated fatty acids (PUFAs) are powerful modulators of several animal ion channels. It is shown here that PUFAs strongly affect the activity of the Slow Vacuolar (SV) channel encoded by the plant TPC1 gene. The patch-clamp technique was applied to isolated vacuoles from carrot taproots and Arabidopsis thaliana mesophyll cells and arachidonic acid (AA) was chosen as a model molecule for PUFAs. Our study was extended to different PUFAs including the endogenous alpha-linolenic acid (ALA). The addition of micromolar concentrations of AA reversibly inhibited the SV channel decreasing the maximum open probability and shifting the half activation voltage to positive values. Comparing the effects of different PUFAs, it was found that the length of the lipophilic acyl chain, the number of double bonds and the polar head were critical for channel modulation.The experimental data can be reproduced by a simple three-state model, in which PUFAs do not interact directly with the voltage sensors but affect the voltage-independent transition that leads the channel from the open state to the closed configuration. The results indicate that lipids play an important role in co-ordinating ion channel activities similar to what is known from animal cells.
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DOI：
10.1073/pnas.93.19.10519
发表时间：
1996-09-17
影响因子：
11.1
作者：
Muchhal, US;Pardo, JM;Raghothama, KG
通讯作者：
Raghothama, KG
影响因子：
7.3
作者：
Ma, Z;Bielenberg, DG;Lynch, JP
通讯作者：
Lynch, JP
影响因子：
2.6
作者：
Lloyd, JC;Zakhleniuk, OV;Raines, CA
通讯作者：
Raines, CA
DOI：
10.1073/pnas.0505266102
发表时间：
2005-08-16
影响因子：
11.1
作者：
Misson, J;Raghothama, KG;Thibaud, MC
通讯作者：
Thibaud, MC
影响因子：
6.4
作者：
MURASHIGE, T;SKOOG, F
通讯作者：
SKOOG, F