An evidence appraisal of heart organoids in a dish and commensurability to human heart development in vivo.
An evidence appraisal of heart organoids in a dish and commensurability to human heart development in vivo.
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DOI：
10.1186/s12872-022-02543-7
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发表时间：
2022-03-22
影响因子：
2.1
通讯作者：
Sayed N
中科院分区：
文献类型：
--
作者：
Thomas D;de Jesus Perez VA;Sayed N
干细胞来源的体外心脏模型已经为心脏发育和疾病的机制提供了深刻的见解。使用三步Wnt信号调制有效地将特定类型的心脏细胞从人类多能干细胞中分化出来是使个性化心血管疾病建模方法成为可能的重大发现之一。心脏细胞类型的产生遵循胚胎发育的关键阶段，它们直观地是研究原始心脏结构中心脏组织模式的优秀模型。在这里，我们简要概述了为干细胞来源的三维心脏模型的建立奠定基础的方案。此外，本文还重点介绍了这些模型的特点和实用性，以区分在对胚胎发育和疾病过程进行建模时的优势和取舍。最后，我们讨论了在当前模型中提高稳健性和利用发展原则带来更高的生理相关性方面的挑战。在体外，人类心脏模型是一种辅助工具，允许以简化论的方式进行机械性询问。利用患者特有的干细胞的独特优势，以及在产生可靠的心脏有机仿制品方面的持续改进，将增强这些工具在基础和翻译研究中的预测能力。
Stem-cell derived in vitro cardiac models have provided profound insights into mechanisms in cardiac development and disease. Efficient differentiation of specific cardiac cell types from human pluripotent stem cells using a three-step Wnt signaling modulation has been one of the major discoveries that has enabled personalized cardiovascular disease modeling approaches. Generation of cardiac cell types follow key development stages during embryogenesis, they intuitively are excellent models to study cardiac tissue patterning in primitive cardiac structures. Here, we provide a brief overview of protocols that have laid the foundation for derivation of stem-cell derived three-dimensional cardiac models. Further this article highlights features and utility of the models to distinguish the advantages and trade-offs in modeling embryonic development and disease processes. Finally, we discuss the challenges in improving robustness in the current models and utilizing developmental principles to bring higher physiological relevance. In vitro human cardiac models are complimentary tools that allow mechanistic interrogation in a reductionist way. The unique advantage of utilizing patient specific stem cells and continued improvements in generating reliable organoid mimics of the heart will boost predictive power of these tools in basic and translational research.
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影响因子：
9.7
作者：
Hazeltine, Laurie B.;Badur, Mehmet G.;Lian, Xiaojun;Das, Amritava;Han, Wenqing;Palecek, Sean P.
通讯作者：
Palecek, Sean P.
影响因子：
4.5
作者：
Amini M;Zayeri F;Salehi M
通讯作者：
Salehi M
影响因子：
--
作者：
Miquerol, Lucile;Kelly, Robert G.
通讯作者：
Kelly, Robert G.
影响因子：
20.1
作者：
Campostrini G;Windt LM;van Meer BJ;Bellin M;Mummery CL
通讯作者：
Mummery CL
影响因子：
14
作者：
Alford, Patrick W.;Feinberg, Adam W.;Sheehy, Sean P.;Parker, Kevin K.
通讯作者：
Parker, Kevin K.