A small conductance calcium-activated K+ channel in C. elegans, KCNL-2, plays a role in the regulation of the rate of egg-laying.
A small conductance calcium-activated K+ channel in C. elegans, KCNL-2, plays a role in the regulation of the rate of egg-laying.
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DOI：
10.1371/journal.pone.0075869
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Luke CJ
中科院分区：
文献类型：
--
作者：
Chotoo CK;Silverman GA;Devor DC;Luke CJ
在小鼠的神经系统中，小电导钙激活钾（SK）通道的功能，以调节神经元的兴奋性，通过产生的一个组成部分的介质后超极化，以下动作电位。在人类中，不规则的动作电位放电频率是共济失调、癫痫、精神分裂症和帕金森病等疾病的基础。由于研究哺乳动物神经系统中蛋白质功能的复杂性，我们试图在C. elegans，一个遗传上易于处理的系统，其中单个神经元的谱系从其早期发育阶段绘制。KCNL-2蛋白的序列分析表明，6个跨膜结构域，钾离子选择性孔和钙调素结合结构域与哺乳动物同源物高度保守。我们使用宽视野和共聚焦荧光成像技术显示，KCNL-2与GFP的融合构建体在转基因株系中在C.优雅我们还表明，KCNL-2无效株，kcnl-2（tm 1885），表现出轻度的产卵缺陷表型，一种表型，是拯救在KCNL-2依赖的方式。相反，我们表明，过表达KCNL-2的转基因株系表现出过度活跃的产卵表型。在这项研究中，我们表明，外阴的转基因雌雄同体是高度神经支配的神经元的过程和VC 4和VC 5神经元表达GFP标记的KCNL-2。我们推测KCNL-2在C.来调节产卵的速度
In the nervous system of mice, small conductance calcium-activated potassium (SK) channels function to regulate neuronal excitability through the generation of a component of the medium afterhyperpolarization that follows action potentials. In humans, irregular action potential firing frequency underlies diseases such as ataxia, epilepsy, schizophrenia and Parkinson’s disease. Due to the complexity of studying protein function in the mammalian nervous system, we sought to characterize an SK channel homologue, KCNL-2, in C. elegans, a genetically tractable system in which the lineage of individual neurons was mapped from their early developmental stages. Sequence analysis of the KCNL-2 protein reveals that the six transmembrane domains, the potassium-selective pore and the calmodulin binding domain are highly conserved with the mammalian homologues. We used widefield and confocal fluorescent imaging to show that a fusion construct of KCNL-2 with GFP in transgenic lines is expressed in the nervous system of C. elegans. We also show that a KCNL-2 null strain, kcnl-2(tm1885), demonstrates a mild egg-laying defective phenotype, a phenotype that is rescued in a KCNL-2-dependent manner. Conversely, we show that transgenic lines that overexpress KCNL-2 demonstrate a hyperactive egg-laying phenotype. In this study, we show that the vulva of transgenic hermaphrodites is highly innervated by neuronal processes and by the VC4 and VC5 neurons that express GFP-tagged KCNL-2. We propose that KCNL-2 functions in the nervous system of C. elegans to regulate the rate of egg-laying.
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