Parasol cells of the hemiellipsoid body in the crayfish Procambarus clarkii: Dendritic branching patterns and functional implications

Parasol cells of the hemiellipsoid body in the crayfish Procambarus clarkii: Dendritic branching patterns and functional implications
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克氏原螯虾半椭圆体伞细胞:树突状分支模式和功能意义

DOI:
10.1002/cne.10716
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发表时间:
2003
影响因子:
2.5
通讯作者:
D. Mellon
D. Mellon
中科院分区:
医学3区
文献类型:
--
作者:
Mary Elizabeth McKinzie;J. Benton;B. Beltz;D. Mellon

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甲壳纲十足类动物的多模式、高阶感觉整合发生在外侧前脑结构内的局部中间神经元(阳伞细胞)中,半椭圆体位于终末延髓的背侧。半椭圆体的目标是通过双侧中脑神经柱,副叶,接受二次视觉,mechanosensory和嗅觉输入的嗅觉球束投射神经元输入。伞细胞树突在半椭圆体的两个神经柱内广泛分支,并且可能在其基部的另一个神经柱内具有一些神经突。两个neuropils的半椭圆体,neuropils I和II,是已知的接收不对称的输入从对侧和同侧的副叶,我们目前的研究解决的问题,在这两个neuropils的伞细胞的分布。三种解剖学方法被用来分析这种分布:细胞内填充的细胞与神经生物素和可视化的细胞,通过使用荧光或过氧化物酶抗生物素蛋白结合物,或放置荧光亲脂示踪剂内的一个叶的半椭圆体。所有这些方法表明,单一的阳伞细胞只在半椭圆体的两个叶之一,但不是在两个叶。电生理记录与树突在相同或不同的叶对阳伞细胞证实了功能的分离neuropils I和II之间。昆虫和甲壳类系统之间的比较,强调输入的hemiellipsoid机构和蘑菇体和外部细胞之间的相似性昆虫和十足类甲壳类动物的阳伞细胞。J. Comp.神经元462:168-179,2003.© 2003 Wiley利斯公司
Multimodal, higher‐order sensory integration in decapod crustaceans occurs in local interneurons (parasol cells) within a structure in the lateral protocerebrum, the hemiellipsoid body, which is located dorsal to the terminal medulla. The hemiellipsoid body is targeted by projection neuron inputs by means of the olfactory globular tract from bilateral deutocerebral neuropils, the accessory lobes, which receive secondary visual, mechanosensory, and olfactory inputs. Parasol cell dendrites arborize extensively within the two neuropils of the hemiellipsoid body and possibly have some neurites within another neuropil at its base. The two neuropils of the hemiellipsoid body, neuropils I and II, are known to receive asymmetrical inputs from the contralateral and ipsilateral accessory lobes, and our current study addresses the question of the distribution of parasol cells within these two neuropils. Three anatomic methods were used to analyze this distribution: intracellular filling of cells with neurobiotin and visualization of the cells by using either a fluorescent or a peroxidase avidin conjugate, or placement of a fluorescent lipophilic tracer within a lobe of the hemiellipsoid body. All of these methods demonstrated that single parasol cells exclusively arborize within one of the two lobes of the hemiellipsoid body, but not in both lobes. Electrophysiological recordings from pairs of parasol cells with dendrites in the same or different lobes confirm a functional separation between neuropils I and II. Comparisons are made between insect and crustacean systems, emphasizing the inputs to the hemiellipsoid body and the mushroom body and similarities between extrinsic cells in insects and parasol cells in decapod crustaceans. J. Comp. Neurol. 462:168–179, 2003. © 2003 Wiley‐Liss, Inc.
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