TRPC1-mediated Ca2+ and Na+ signalling in astroglia: differential filtering of extracellular cations.
TRPC1-mediated Ca2+ and Na+ signalling in astroglia: differential filtering of extracellular cations.
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DOI：
10.1016/j.ceca.2013.05.005
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发表时间：
2013-08
期刊：
影响因子：
4
通讯作者：
Parpura V
中科院分区：
文献类型：
--
作者：
Reyes RC;Verkhratsky A;Parpura V
经典瞬时受体电位1（TRPC 1）质膜阳离子通道介导大鼠皮质星形胶质细胞内Ca ~（2+）和Na ~+的流动，并控制各自的胞浆离子信号。星形胶质细胞的机械刺激导致细胞溶质Ca 2+和Na+水平的增加，这部分是由于细胞外阳离子通过含TRPC 1的通道进入。用针对TRPC 1的选择性过滤器的抗体抑制TRPC 1孔减少了由机械刺激引起的细胞溶质Ca 2+积累。相反，这种免疫治疗增加了机械刺激诱导的细胞溶质Na+峰积累。我们建议，TRPC通道是适合在选择性过滤的变化，在以前的研究和抗体结合在我们目前的研究中的突变差异影响流量的Ca 2+和Na+。TRPC 1通道可能代表协调的Ca 2+和Na+信号在星形胶质细胞的焦点，这可以有后果的Ca 2+和Na+依赖的过程，如调节胞吐和乳酸的生产，分别，这反过来又可以调节神经元突触传递。
Canonical transient receptor potential 1 (TRPC1) plasmalemmal cation channels mediate Ca2+ and Na+ fluxes and control respective cytoplasmic ion signals in rat cortical astrocytes. Mechanical stimulation of astrocytes results in increases in cytosolic Ca2+ and Na+ levels that are in part due to entry of extracellular cations through TRPC1 containing channels. Inhibition of the TRPC1 pore with an antibody against the selective filter of TRPC1 reduced cytosolic Ca2+ accumulation caused by mechanical stimulation. In contrast, this immunological treatment increased the cytosolic Na+ peak accumulation induced by mechanical stimulation. We propose that TRPC channels are amenable to changes in selective filtering as mutations in previous studies and antibody binding in our present study differentially affect the flux of Ca2+ and Na+. TRPC1 containing channels might represent focal points for co-ordination of Ca2+ and Na+ signalling in astroglia and this can have consequences on Ca2+- and Na+-dependent processes such as regulated exocytosis and lactate production, respectively, which in turn can modulate neuronal synaptic transmission.
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影响因子：
5.3
作者：
Montana, V;Ni, YC;Parpura, V
通讯作者：
Parpura, V
DOI：
10.1016/j.bbamcr.2010.11.006
发表时间：
2011-05-01
影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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影响因子：
16.2
作者：
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通讯作者：
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影响因子：
3
作者：
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通讯作者：
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